Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy

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Data

2002-01-01

Autores

Lima, Ana Silvia Gouvêa
Guidelli, Ana Maria [UNESP]
Abreu, Irlan Leite de [UNESP]
Lemos, Manuel Victor Franco [UNESP]

Título da Revista

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Título de Volume

Editor

Sociedade Brasileira de Genética

Resumo

PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.

Descrição

Palavras-chave

REP, ERIC, crystal protein, parasporal bodies

Como citar

Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 25, n. 2, p. 225-229, 2002.

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