Crystallization and preliminary X-ray diffraction studies of BmooPLA(2)-I, a platelet-aggregation inhibitor and hypotensive phospholipase A(2) from Bothrops moojeni venom

Página do item simplificado
dc.contributor.authorSalvador, Guilherme H. M. [UNESP]
dc.contributor.authorMarchi-Salvador, Daniela P. [UNESP]
dc.contributor.authorSilveira, Lucas B.
dc.contributor.authorSoares, Andreimar M.
dc.contributor.authorFontes, Marcos R. M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de Rondônia (UNIR)
dc.date.accessioned2014-05-20T13:49:40Z
dc.date.available2014-05-20T13:49:40Z
dc.date.issued2011-08-01
dc.description.abstractPhospholipases A(2) (PLA(2)s) are enzymes that cause the liberation of fatty acids and lysophospholipids by the hydrolysis of membrane phospholipids. In addition to their catalytic action, a wide variety of pharmacological activities have been described for snake-venom PLA(2)s. BmooPLA(2)-I is an acidic, nontoxic and catalytic PLA(2) isolated from Bothrops moojeni snake venom which exhibits an inhibitory effect on platelet aggregation, an immediate decrease in blood pressure, inducing oedema at a low concentration, and an effective bactericidal effect. BmooPLA(2)-I has been crystallized and X-ray diffraction data have been collected to 1.6 angstrom resolution using a synchrotron-radiation source. The crystals belonged to space group C222(1), with unit-cell parameters a = 39.7, b = 53.2, c = 89.2 angstrom. The molecular-replacement solution of BmooPLA(2)-I indicated a monomeric conformation, which is in agreement with nondenaturing electrophoresis and dynamic light-scattering experiments. A comparative study of this enzyme with the acidic PLA(2) from B. jararacussu (BthA-I) and other toxic and nontoxic PLA(2)s may provide important insights into the functional aspects of this class of proteins.en
dc.description.affiliationUNESP Univ Estadual Paulista, Inst Biociencias, Dept Fis & Biofis, Botucatu, SP, Brazil
dc.description.affiliationCNPq, Inst Nacl Ciência & Tecnol Toxinas, Brasilia, DF, Brazil
dc.description.affiliationUniv São Paulo, FCFRP, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Fed Rondonia, Fundação Oswaldo Cruz, FIOCRUZ Rondonia, Porto Velho, RO, Brazil
dc.description.affiliationUniv Fed Rondonia, Ctr Estudos Biomol Aplicadas CEBio, Porto Velho, RO, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Inst Biociencias, Dept Fis & Biofis, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipLaboratório Nacional de Luz Síncrotron (LNLS)
dc.format.extent900-902
dc.identifierhttp://dx.doi.org/10.1107/S174430911102392X
dc.identifier.citationActa Crystallographica Section F-structural Biology and Crystallization Communications. Hoboken: Wiley-blackwell, v. 67, p. 900-902, 2011.
dc.identifier.doi10.1107/S174430911102392X
dc.identifier.fileWOS000293698400013.pdf
dc.identifier.issn1744-3091
dc.identifier.urihttp://hdl.handle.net/11449/17707
dc.identifier.wosWOS:000293698400013
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofActa Crystallographica Section F: Structural Biology and Crystallization Communications
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleCrystallization and preliminary X-ray diffraction studies of BmooPLA(2)-I, a platelet-aggregation inhibitor and hypotensive phospholipase A(2) from Bothrops moojeni venomen
dc.typeArtigo
dcterms.licensehttp://journals.iucr.org/services/copyrightpolicy.html
dcterms.rightsHolderWiley-blackwell
unesp.author.orcid0000-0002-4634-6221[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000293698400013.pdf
Tamanho:
166.55 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Física e Biofísica - IBB