Development of fibronectin-loaded nanofiber scaffolds for guided pulp tissue regeneration

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dc.contributor.authorLeite, Maria Luisa [UNESP]
dc.contributor.authorSoares, Diana Gabriela
dc.contributor.authorAnovazzi, Giovana [UNESP]
dc.contributor.authorMendes Soares, Igor Paulino [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorSouza Costa, Carlos Alberto de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2021-06-25T12:30:46Z
dc.date.available2021-06-25T12:30:46Z
dc.date.issued2020-12-31
dc.description.abstractFibronectin (FN)-loaded nanofiber scaffolds were developed and assessed concerning their bioactive potential on human apical papilla cells (hAPCs). First, random (NR) and aligned (NA) nanofiber scaffolds of polycaprolactone (PCL) were obtained by electrospinning technique and their biological properties were evaluated. The best formulations of NR and NA were loaded with 0, 5, or 10 mu g/ml of FN and their bioactivity was assessed. Finally, FN-loaded NR and NA tubular scaffolds were prepared and their chemotactic potential was analyzed using an in vitro model to mimic the pulp regeneration of teeth with incomplete root formation. All scaffolds tested were cytocompatible. However, NR and NA based on 10% PCL promoted the highest hAPCs proliferation, adhesion and spreading. Polygonal and elongated cells were observed on NR and NA, respectively. The higher the concentration of FN added to the scaffolds, greater cell migration, viability, proliferation, adhesion and spreading, as well as collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1). In addition, tubular scaffolds with NA loaded with FN (10 mu g/ml) showed the highest chemotactic potential on hAPCs. It was concluded that FN-loaded NA scaffolds may be an interesting biomaterial to promote hAPCs-mediated pulp regeneration of endodontically compromised teeth with incomplete root formation.en
dc.description.affiliationUniv Estadual Paulista, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru, SP, Brazil
dc.description.affiliationSao Paulo State Univ, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, SP, Brazil
dc.description.affiliationSao Paulo State Univ, Araraquara Sch Dent, Dept Physiol & Pathol, Humaita St 1680, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Araraquara Sch Dent, Dept Physiol & Pathol, Humaita St 1680, BR-14801903 Araraquara, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 302047/2019-0
dc.description.sponsorshipIdCNPq: 408721/2018-9
dc.description.sponsorshipIdFAPESP: 2017/14210-8
dc.description.sponsorshipIdFAPESP: 2017/22739-9
dc.format.extent15
dc.identifierhttp://dx.doi.org/10.1002/jbm.b.34785
dc.identifier.citationJournal Of Biomedical Materials Research Part B-applied Biomaterials. Hoboken: Wiley, 15 p., 2020.
dc.identifier.doi10.1002/jbm.b.34785
dc.identifier.issn1552-4973
dc.identifier.urihttp://hdl.handle.net/11449/209834
dc.identifier.wosWOS:000603646000001
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal Of Biomedical Materials Research Part B-applied Biomaterials
dc.sourceWeb of Science
dc.subjectapical papilla cells
dc.subjectfibronectin
dc.subjectnanofibers
dc.subjectpulp regeneration
dc.subjecttissue engineering
dc.titleDevelopment of fibronectin-loaded nanofiber scaffolds for guided pulp tissue regenerationen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.orcid0000-0002-5748-5040[4]

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Coleções

Artigos - Materiais Odontológicos e Prótese - FOAR
Artigos - Clínica Infantil - FOAR
Artigos - Fisiologia e Patologia - FOAR