Identification of Biomarkers of Mercury Contamination in Brachyplatystoma filamentosum of the Madeira River, Brazil, Using Metalloproteomic Strategies

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dc.contributor.authorde Queiroz, João Vitor [UNESP]
dc.contributor.authorVieira, José Cavalcante Souza [UNESP]
dc.contributor.authorde Oliveira, Grasieli [UNESP]
dc.contributor.authorBraga, Camila Pereira
dc.contributor.authorda Cunha Bataglioli, Izabela [UNESP]
dc.contributor.authorda Silva, Janaína Macedo [UNESP]
dc.contributor.authorde Paula Araújo, Wellington Luiz [UNESP]
dc.contributor.authorde Magalhães Padilha, Pedro [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Nebraska
dc.date.accessioned2018-12-11T16:53:12Z
dc.date.available2018-12-11T16:53:12Z
dc.date.issued2018-05-08
dc.description.abstractPredator fish can accumulate high levels of mercury, which qualifies them as potential indicators of this toxic metal. The predatory species Brachyplatystoma filamentosum, popularly known as filhote, is among the most consumed species in the Brazilian Amazon. Continuing the metalloproteomic studies of mercury in Amazonian fishes that have been developed in the last 5 years, the present paper provides the data of protein characterization associated with mercury in muscle and liver samples of filhote (Brachyplatystoma filamentosum) collected in the Madeira River, Brazilian Amazon. The mercury concentration in the muscle and liver samples was determined by graphite furnace atomic absorption spectrometry (GFAAS). The protein fraction was extracted in an aqueous medium, and later, a fractional precipitation procedure was performed to obtain the protein pellets. Then, the proteome of the tissue samples of this fish species was separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and a mercury mapping of the protein spots was carried out by GFAAS after acid digestion. Protein spots that had mercury were characterized by mass spectrometry with electrospray ionization in sequence (ESI-MS/MS) after tryptic digestion. It was possible to characterize 11 mercury-associated protein spots that presented biomarker characteristics and could be used to monitor mercury in fish species of the Amazon region. Thus, the metalloproteomic strategies used in the present study allowed us to characterize 11 mercury-associated protein spots. It should be noted that the protein spots identified as GFRP, TMEM186, TMEM57B, and BHMT, which have coordination sites for elements with characteristics of soft acids, such as mercury, can be used as biomarkers of mercury contamination in monitoring studies of this toxic metal in fish species from the Amazon region.en
dc.description.affiliationSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
dc.description.affiliationInstitute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationBiochemistry Department University of Nebraska
dc.description.affiliationUnespSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
dc.description.affiliationUnespInstitute of Biosciences São Paulo State University (UNESP)
dc.format.extent1-10
dc.identifierhttp://dx.doi.org/10.1007/s12011-018-1363-5
dc.identifier.citationBiological Trace Element Research, p. 1-10.
dc.identifier.doi10.1007/s12011-018-1363-5
dc.identifier.file2-s2.0-85046698487.pdf
dc.identifier.issn1559-0720
dc.identifier.issn0163-4984
dc.identifier.scopus2-s2.0-85046698487
dc.identifier.urihttp://hdl.handle.net/11449/170976
dc.language.isoeng
dc.relation.ispartofBiological Trace Element Research
dc.relation.ispartofsjr0,719
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBiomarkers
dc.subjectMercury
dc.subjectProteins
dc.subjectProteomics
dc.subjectSystem biology
dc.titleIdentification of Biomarkers of Mercury Contamination in Brachyplatystoma filamentosum of the Madeira River, Brazil, Using Metalloproteomic Strategiesen
dc.typeArtigo

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