Effect of combining Zingiber officinale and Juglans regia extracts on Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis: antibiofilm action and low toxicity

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dc.contributor.authorDa Silva, Leonardo A. [UNESP]
dc.contributor.authorRamos, Lucas P. [UNESP]
dc.contributor.authorSilva, Tatiane A. [UNESP]
dc.contributor.authorDe Lapena, Simone A. B. [UNESP]
dc.contributor.authorSantos, Carlos Eduardo R. [UNESP]
dc.contributor.authorHasna, Amjad A. [UNESP]
dc.contributor.authorBressane, Adriano [UNESP]
dc.contributor.authorDe oliveira, Luciane D. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionInstituto Taubaté de Ensino Superior/ITES
dc.contributor.institutionInstituto Policlin de Ensino e Pesquisas/IPEP
dc.date.accessioned2023-07-29T13:30:47Z
dc.date.available2023-07-29T13:30:47Z
dc.date.issued2022-01-01
dc.description.abstractObjective was evaluated the therapeutic effect of Juglans regia (J) and Zingiber officinale (Z) extracts, alone or associated (Z75% + J25%, Z50% + J50% and Z25% + J75%) applied on planktonic cultures and biofilms of Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus, as well as analyzing the cytotoxic effects of plant extracts on mouse macrophages (Raw 264-7). Broth microdilution assay was performed (M7-A6-CLSI). Anti-biofilm activities and cytotoxicity on Raw 264-7 were studied using MTT assay and scanning electron microscopy. ANOVA with post-hoc Tukey HSD applied for parametric data and Kruskal-Wallis with Conover-Iman test, for non-parametric (p<0.05). On P. acnes biofilm, Z50% + J50% reduced 46.9% in 5 min and Z25% + J75% reduced 74.1% in 24hs. On S. aureus, Z75% + J25% reduced 23.1% in 5 min Z25% +J75% reduced 79.4% in 24hs. On S. epidermidis, Z75% + J25% reduced 74.6% in 5 min and 82.05% in 24 h. The treatments on macrophages for 24 h promoted a maximum reduction by 14,5% for groups of extracts associations. On multispecies biofilm, Z75%+J25% reduced 84.3% in 24 h. In conclusion association of glycolic extracts provided therapeutic effect, demonstrated antimicrobial activity and low cytotoxicity.en
dc.description.affiliationUniversidade Estadual Paulista/ UNESP Instituto de Ciencia e Tecnologia Departamento de Biociências e Diagnóstico Bucal, Av. Eng. Francisco José Longo, 777, São Dimas, SP
dc.description.affiliationInstituto Taubaté de Ensino Superior/ITES Departamento de Ciências da Saúde, Av. Dom Pedro I, 3575, Jardim Eulália, SP
dc.description.affiliationInstituto Policlin de Ensino e Pesquisas/IPEP, Av. Nove de Julho, 430, Vila Ady’Anna, SP
dc.description.affiliationUnespUniversidade Estadual Paulista/ UNESP Instituto de Ciencia e Tecnologia Departamento de Biociências e Diagnóstico Bucal, Av. Eng. Francisco José Longo, 777, São Dimas, SP
dc.identifierhttp://dx.doi.org/10.1590/0001-3765202220201133
dc.identifier.citationAnais da Academia Brasileira de Ciencias, v. 94, n. 4, 2022.
dc.identifier.doi10.1590/0001-3765202220201133
dc.identifier.issn1678-2690
dc.identifier.issn0001-3765
dc.identifier.scopus2-s2.0-85142911582
dc.identifier.urihttp://hdl.handle.net/11449/247967
dc.language.isoeng
dc.relation.ispartofAnais da Academia Brasileira de Ciencias
dc.sourceScopus
dc.subjectAcne Vulgaris
dc.subjectanti-infective agents
dc.subjectdermatologic agents
dc.subjectJuglans regia
dc.subjecttoxicity
dc.subjectZingiber officinale
dc.titleEffect of combining Zingiber officinale and Juglans regia extracts on Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis: antibiofilm action and low toxicityen
dc.typeArtigo
unesp.author.orcid0000-0002-7565-969X[1]
unesp.author.orcid0000-0002-2682-2796[2]
unesp.author.orcid0000-0002-5499-5299[3]
unesp.author.orcid0000-0001-9790-3027[4]
unesp.author.orcid0000-0001-6592-0244[5]
unesp.author.orcid0000-0002-1112-985X[6]
unesp.author.orcid0000-0002-5465-9551[8]

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Artigos - Biociências e Diagnóstico Bucal - ICT