Determination of pantoprazole in human plasma by LC-MS-MS using lansoprazole as internal standard

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dc.contributor.authorPeres, O.
dc.contributor.authorOliveira, C. H.
dc.contributor.authorBarrientos-Astigarraga, R. E.
dc.contributor.authorRezende, V. M.
dc.contributor.authorMendes, G. D.
dc.contributor.authorNucci, G. de
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:54:19Z
dc.date.available2015-03-18T15:54:19Z
dc.date.issued2004-01-01
dc.description.abstractAn analytical method based on liquid chromatography with positive ion electrospray ionization (ESI) coupled to tandem mass spectrometry detection was developed for the determination of pantoprazole (CAS 102625-70-7) in human plasma using lansoprazole (CAS 103577-45-3) as the internal standard. The analyte and internal standard were extracted from the plasma samples by liquid/liquid extraction using diethyl-ether/dichloromethane (70:30; v/v) and chromatographed on a C-8 analytical column. The mobile phase consisted of acetonitrile/water/methanol (57:25:18; v/v/v) + 10 mmol/l acetic acid + 20 mmol/l ammonium acetate. The method has a chromatographic total run time of 4.5 min and was linear within the range 5.0-5,000 ng/mL. Detection was performed on a triple quadrupole tandem mass spectrometer by Multiple Reaction Monitoring (MRM). The intra- and inter-run precisions calculated from quality control (QC) samples were 4.2% and 3.2%, respectively. The accuracies as determined from QC samples were -5.0% (intra-run) and 2.0% (inter-run). The method herein described was employed in a bioequivalence study of two tablet formulations of pantoprazole.en
dc.description.affiliationUniv Estadual Campinas, Dept Pharmacol, Campinas, SP, Brazil
dc.description.affiliationState Univ Sao Paulo, Inst Biol Sci, Dept Pharmacol, Cartesius Analyt Unit, Sao Paulo, Brazil
dc.description.affiliationUnespState Univ Sao Paulo, Inst Biol Sci, Dept Pharmacol, Cartesius Analyt Unit, Sao Paulo, Brazil
dc.format.extent314-319
dc.identifierhttp://dx.doi.org/10.1055/s-0031-1296977
dc.identifier.citationArzneimittel-forschung-drug Research. Aulendorf: Ecv-editio Cantor Verlag Medizin Naturwissenschaften, v. 54, n. 6, p. 314-319, 2004.
dc.identifier.doi10.1055/s-0031-1296977
dc.identifier.issn0004-4172
dc.identifier.urihttp://hdl.handle.net/11449/116876
dc.identifier.wosWOS:000222617400002
dc.language.isoeng
dc.publisherEcv-editio Cantor Verlag Medizin Naturwissenschaften
dc.relation.ispartofArzneimittel-forschung-drug Research
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCAS 102625-70-7en
dc.subjectpantoprazole, determination in human plasmaen
dc.subjectproton pump inhibitoren
dc.titleDetermination of pantoprazole in human plasma by LC-MS-MS using lansoprazole as internal standarden
dc.typeArtigo
dcterms.rightsHolderEcv-editio Cantor Verlag Medizin Naturwissenschaften
unesp.author.orcid0000-0002-6586-0496[4]
unesp.author.orcid0000-0002-4346-7941[6]

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