Chemopreventive Activity of Systemically Administered Curcumin on Oral Cancer in the 4-Nitroquinoline 1-Oxide Model

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dc.contributor.authorGoncalves, Vinicius de Paiva [UNESP]
dc.contributor.authorOrtega, Adriana Alicia C. [UNESP]
dc.contributor.authorGuimaraes, Morgana R. [UNESP]
dc.contributor.authorCurylofo, Fabiana Almeida [UNESP]
dc.contributor.authorRossa Junior, Carlos [UNESP]
dc.contributor.authorRibeiro, Daniel Araki
dc.contributor.authorSpolidorio, Luis C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-10-21T13:10:41Z
dc.date.available2015-10-21T13:10:41Z
dc.date.issued2015-05-01
dc.description.abstractCurcumin has therapeutic potential in preventing several types of cancer, including colon, liver, prostate, and breast. The goal of this study was to evaluate the chemopreventive activity of systemically administered curcumin on oral carcinogenesis induced by 4-nitroquinolone-1-oxide (4-NQO). A total of 50 male albino rats, Rattus norvegicus, (Holtzman), were divided into five groups (n = 10 per group). Four of these groups were exposed to 50 ppm 4-NQO in their drinking water ad libitum for 8 or 12 weeks, two groups were treated with curcumin by oral gavage at 30 or 100 mg/kg per day, and one group was treated with corn oil (vehicle) only. The negative control group was euthanized at baseline. Tongues of all animals were removed after euthanasia and used in the subsequent analysis because the tongue is the primary site of carcinogenesis in this model. Descriptive histological analysis and immunohistochemistry for PCNA, Bcl-2, SOCS1 e-3, and STAT3 were performed to assess the oncogenic process. The gene expression of Vimentin, E-cadherin, N-cadherin, or TWIST1 was assessed using RT-qPCR as a representative of epithelial-mesenchymal transition (EMT) events. The administration of curcumin at 100 mg/kg during the 12 weeks markedly decreased the expression of PCNA, Bcl-2, SOCS1 e-3, and STAT3. Curcumin also minimized the cellular atypia under microscopic analysis and diminished the expression of the genes associated with EMT. These findings demonstrate that the systemic administration of curcumin has chemopreventive activity during oral carcinogenesis induced by 4-NQO. J. Cell. Biochem. 116: 787-796, 2015. (C) 2014 Wiley Periodicals, Inc.en
dc.description.affiliationUniv Sao Paulo State UNESP, Araraquara Sch Dent, Dept Diag &Surg, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Dept Biosci, Santos, SP, Brazil
dc.description.affiliationUniv Sao Paulo State UNESP, Araraquara Sch Dent, Dept Physiol &Pathol, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Sao Paulo State UNESP, Araraquara Sch Dent, Dept Diag &Surg, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Sao Paulo State UNESP, Araraquara Sch Dent, Dept Physiol &Pathol, BR-14801903 Araraquara, SP, Brazil
dc.format.extent787-796
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1002/jcb.25035/abstract
dc.identifier.citationJournal Of Cellular Biochemistry. Hoboken: Wiley-blackwell, v. 116, n. 5, p. 787-796, 2015.
dc.identifier.doi10.1002/jcb.25035
dc.identifier.issn0730-2312
dc.identifier.urihttp://hdl.handle.net/11449/128529
dc.identifier.wosWOS:000352817300011
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal Of Cellular Biochemistry
dc.relation.ispartofjcr2.959
dc.relation.ispartofsjr1,209
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subject4-NITROQUINOLINE-1-OXIDEen
dc.subjectRATen
dc.subjectTONGUEen
dc.subjectCURCUMINen
dc.subjectCHEMOPREVENTIONen
dc.titleChemopreventive Activity of Systemically Administered Curcumin on Oral Cancer in the 4-Nitroquinoline 1-Oxide Modelen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes7634063102292261[5]
unesp.author.orcid0000-0003-1705-5481[5]
unesp.author.orcid0000-0002-0482-6956[4]
unesp.author.orcid0000-0002-1297-9717[3]
unesp.author.orcid0000-0002-0592-542X[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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