Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines

dc.contributor.authorPagani Possamai, Mari Helen
dc.contributor.authorBattilana, Jaqueline
dc.contributor.authorPaludo, Ediane
dc.contributor.authorHerkenhoff, Marcos Edgar [UNESP]
dc.contributor.authorPertille, Fabio
dc.contributor.authorLima-Rosa, Carlos Andre da Veiga
dc.contributor.institutionUniversidade de Estado de Santa Catarina (UDESC)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-10-21T13:11:17Z
dc.date.available2015-10-21T13:11:17Z
dc.date.issued2015-05-01
dc.description.abstractThis study aimed to investigate the genetic variability of two Brazilian free range (Caipira) chickens lines using microsatellites analysis of ten loci. It was collected a total of 99 blood samples, which 49 were from Paraiso Pedres (PP) and 50 were from Rubro Negra (RN) lines. The amplification of the DNA fragments was performed by polymerase chain reaction (PCR) and the genotyping was conduct using ABI 3130 sequencer. The allele number variation was among 3 (LEI0254) to 32 (LEI0212) in the PP line, and 4 (LEI0254) to 31 (LEI0212) in the RN line. The allelic average per locus was 13.3 and 13.1 in the PP and RN lines, respectively. The average observed and the expected heterozygosity were 0.650 and 0.820 in the PP line, and 0.671 and 0.804 in the RN line. All of the analyzed loci were informative (PIC>0.5). These results indicate that these free-range animals have a high genetic variability, at least for the majority of the analyzed loci, and this genetic variation is higher than the commercial chickens and similar for the no-commercial birds.en
dc.description.affiliationUniv Estado Santa Catarina UDESC, Ctr Ciencias Agrovet CAV, DNA UDESC, Lab Anal Genet, Lages, SC, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Inst Biociencias, Dept Genet, Botucatu, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Escola Super Agr Luiz de Queiroz ESALQ, Lab Biotecnol, Piracicaba, SP, Brazil
dc.description.affiliationUDESC, Ctr Educ Super Regiao Sul CERES, Lab Genet Mol LAGENMOL, BR-88790000 Laguna, SC, Brazil
dc.description.affiliationUnespUniversidade Estadual Paulista, UNESP, Inst Biociencias, Dept Genet, Botucatu, SP, Brazil
dc.format.extent877-883
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500877&lng=en&nrm=iso&tlng=en
dc.identifier.citationCiencia Rural, v. 45, n. 5, p. 877-883, 2015.
dc.identifier.doi10.1590/0103-8478cr20140132
dc.identifier.fileS0103-84782015000500877.pdf
dc.identifier.issn0103-8478
dc.identifier.scieloS0103-84782015000500877
dc.identifier.urihttp://hdl.handle.net/11449/128590
dc.identifier.wosWOS:000354789100021
dc.language.isoeng
dc.publisherUniv Federal Santa Maria
dc.relation.ispartofCiencia Rural
dc.relation.ispartofjcr0.525
dc.relation.ispartofsjr0,337
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subject(Caipira) broiler lineen
dc.subject(Caipira) layer lineen
dc.subjectMicrosatelliteen
dc.subjectPolymorphismen
dc.titleGenotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken linesen
dc.typeArtigo
dcterms.rightsHolderUniv Federal Santa Maria
unesp.author.orcid0000-0002-7214-9184[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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