Assessment of ALA-induced PpIX production in porcine skin pretreated with microneedles
dc.contributor.author | Rodrigues, Phamilla Gracielli Sousa | |
dc.contributor.author | Campos de Menezes, Priscila Fernanda | |
dc.contributor.author | Fujita, Alessandra Keiko Lima | |
dc.contributor.author | Escobar, André [UNESP] | |
dc.contributor.author | Barboza de Nardi, Andrigo [UNESP] | |
dc.contributor.author | Kurachi, Cristina | |
dc.contributor.author | Bagnato, Vanderlei S. | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2015-12-07T15:32:19Z | |
dc.date.available | 2015-12-07T15:32:19Z | |
dc.date.issued | 2015 | |
dc.description.abstract | Photodynamic therapy (PDT) is used for skin treatments of premalignant and cancer lesions and recognized as a non-invasive technique that combines tissue photosensitization and subsequent exposure to light to induce cell death. However, it is limited to the treatment of superficial lesions, mainly due to the low cream penetration. Therefore, the improvement of transdermal distribution of aminolevulinic acid (ALA) is needed. In this study, the kinetics and homogeneity of production of ALA-induced PpIX after the skin pre-treatment with microneedles rollers of 0.5, 1.0 and 1.5 mm length were investigated. An improvement in homogeneity and production of PpIX was shown in a porcine model. Widefield fluorescence imaging three hours after the topical application of ALA-cream in the combined treatment with microeedles rollers. | en |
dc.description.affiliation | Sao Carlos Institute of Physics, University of Sao Paulo (USP), Biophotonics Laboratory, Sao Carlos-SP, Brazil. phamilla@ymail.com. | |
dc.description.affiliation | Sao Carlos Institute of Physics, University of Sao Paulo (USP), Biophotonics Laboratory, Sao Carlos-SP, Brazil. | |
dc.description.affiliation | Department of Clinical and Veterinary Surgery, São Paulo State University (UNESP), Jaboticabal-SP, Brazil. | |
dc.description.affiliationUnesp | Department of Clinical and Veterinary Surgery, São Paulo State University (UNESP), Jaboticabal-SP, Brazil. | |
dc.format.extent | 723-729 | |
dc.identifier | http://dx.doi.org/10.1002/jbio.201400081 | |
dc.identifier.citation | Journal Of Biophotonics, v. 8, n. 9, p. 723-729, 2015. | |
dc.identifier.doi | 10.1002/jbio.201400081 | |
dc.identifier.issn | 1864-0648 | |
dc.identifier.pubmed | 25319567 | |
dc.identifier.uri | http://hdl.handle.net/11449/131178 | |
dc.language.iso | eng | |
dc.publisher | 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | |
dc.relation.ispartof | Journal Of Biophotonics | |
dc.rights.accessRights | Acesso restrito | |
dc.source | PubMed | |
dc.subject | Ppix production | en |
dc.subject | Aminolevulinic acid | en |
dc.subject | Microneedle rollers | en |
dc.subject | Photodynamic therapy | en |
dc.subject | Porcine skin | en |
dc.title | Assessment of ALA-induced PpIX production in porcine skin pretreated with microneedles | en |
dc.type | Artigo | |
dcterms.rightsHolder | © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.© 2014 Wiley-vch Verlag Gmbh & Co. Kgaa, Weinheim. | |
unesp.author.lattes | 5256503293611165[5] | |
unesp.author.orcid | 0000-0001-6463-2144[5] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal | pt |
unesp.department | Clínica e Cirurgia Veterinária - FCAV | pt |