Assessment of ALA-induced PpIX production in porcine skin pretreated with microneedles

dc.contributor.authorRodrigues, Phamilla Gracielli Sousa
dc.contributor.authorCampos de Menezes, Priscila Fernanda
dc.contributor.authorFujita, Alessandra Keiko Lima
dc.contributor.authorEscobar, André [UNESP]
dc.contributor.authorBarboza de Nardi, Andrigo [UNESP]
dc.contributor.authorKurachi, Cristina
dc.contributor.authorBagnato, Vanderlei S.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-07T15:32:19Z
dc.date.available2015-12-07T15:32:19Z
dc.date.issued2015
dc.description.abstractPhotodynamic therapy (PDT) is used for skin treatments of premalignant and cancer lesions and recognized as a non-invasive technique that combines tissue photosensitization and subsequent exposure to light to induce cell death. However, it is limited to the treatment of superficial lesions, mainly due to the low cream penetration. Therefore, the improvement of transdermal distribution of aminolevulinic acid (ALA) is needed. In this study, the kinetics and homogeneity of production of ALA-induced PpIX after the skin pre-treatment with microneedles rollers of 0.5, 1.0 and 1.5 mm length were investigated. An improvement in homogeneity and production of PpIX was shown in a porcine model. Widefield fluorescence imaging three hours after the topical application of ALA-cream in the combined treatment with microeedles rollers.en
dc.description.affiliationSao Carlos Institute of Physics, University of Sao Paulo (USP), Biophotonics Laboratory, Sao Carlos-SP, Brazil. phamilla@ymail.com.
dc.description.affiliationSao Carlos Institute of Physics, University of Sao Paulo (USP), Biophotonics Laboratory, Sao Carlos-SP, Brazil.
dc.description.affiliationDepartment of Clinical and Veterinary Surgery, São Paulo State University (UNESP), Jaboticabal-SP, Brazil.
dc.description.affiliationUnespDepartment of Clinical and Veterinary Surgery, São Paulo State University (UNESP), Jaboticabal-SP, Brazil.
dc.format.extent723-729
dc.identifierhttp://dx.doi.org/10.1002/jbio.201400081
dc.identifier.citationJournal Of Biophotonics, v. 8, n. 9, p. 723-729, 2015.
dc.identifier.doi10.1002/jbio.201400081
dc.identifier.issn1864-0648
dc.identifier.pubmed25319567
dc.identifier.urihttp://hdl.handle.net/11449/131178
dc.language.isoeng
dc.publisher2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
dc.relation.ispartofJournal Of Biophotonics
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectPpix productionen
dc.subjectAminolevulinic aciden
dc.subjectMicroneedle rollersen
dc.subjectPhotodynamic therapyen
dc.subjectPorcine skinen
dc.titleAssessment of ALA-induced PpIX production in porcine skin pretreated with microneedlesen
dc.typeArtigo
dcterms.rightsHolder© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.© 2014 Wiley-vch Verlag Gmbh & Co. Kgaa, Weinheim.
unesp.author.lattes5256503293611165[5]
unesp.author.orcid0000-0001-6463-2144[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentClínica e Cirurgia Veterinária - FCAVpt

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