Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7

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dc.contributor.authorJorjão, Adeline Lacerda [UNESP]
dc.contributor.authorDe Oliveira, Felipe Eduardo [UNESP]
dc.contributor.authorLeão, Mariella Vieira Pereira
dc.contributor.authorCarvalho, Cláudio Antonio Talge [UNESP]
dc.contributor.authorJorge, Antonio Olavo Cardoso [UNESP]
dc.contributor.authorDe Oliveira, Luciane Dias [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Taubaté
dc.date.accessioned2018-12-11T16:59:23Z
dc.date.available2018-12-11T16:59:23Z
dc.date.issued2015-01-01
dc.description.abstractThis study aimed to evaluate the capacity of Lactobacillus rhamnosus and/or its products to induce the synthesis of cytokines (TNF-α, IL-1β, IL-4, IL-6, IL-10, and IL-12) by mouse macrophages (RAW 264.7). Three microorganism preparations were used: live L. rhamnosus (LLR) suspension, heat-killed L. rhamnosus (HKLR) suspension, and the supernatant of a heat-killed L. rhamnosus (SHKLR) suspension, which were cultured with macrophages (37°C, 5% CO2) for 2 h and 30 min. After that, cells were cultured for 16 h. The supernatants were used for the quantitation of cytokines, by ELISA. The results were compared with the synthesis induced by lipopolysaccharide (LPS) and analysed, using ANOVA and Tukey test, 5%. LLR and HKLR groups were able to significantly increase the production of TNF-α, IL-6, and IL-10 (P < 0. 05). SHKLR also significantly increased the production of TNF-α and IL-10 (P < 0. 05) but not IL-6 (P > 0. 05). All the L. rhamnosus suspensions were not able to produce detectable levels of IL-1β or significant levels of IL-4 and IL-12 (P > 0. 05). In conclusion, live and heat-killed L. rhamnosus suspensions were able to induce the synthesis of different cytokines with proinflammatory (TNF-α and IL-6) or regulatory (IL-10) functions, suggesting the role of strain L. rhamnosus ATCC 7469 in the modulation or in the stimulation of immune responses.en
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis Laboratory of Microbiology and Immunology Institute of Science and Technology Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo
dc.description.affiliationBioscience Basic Institute University of Taubaté, Street Expedicionário Ernesto Pereira 3
dc.description.affiliationDepartment of Restorative Dentistry Institute of Science and Technology Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis Laboratory of Microbiology and Immunology Institute of Science and Technology Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo
dc.description.affiliationUnespDepartment of Restorative Dentistry Institute of Science and Technology Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo
dc.identifierhttp://dx.doi.org/10.1155/2015/716749
dc.identifier.citationScientific World Journal, v. 2015.
dc.identifier.doi10.1155/2015/716749
dc.identifier.file2-s2.0-84948845306.pdf
dc.identifier.issn1537-744X
dc.identifier.issn2356-6140
dc.identifier.lattes9304600166583100
dc.identifier.orcid0000-0003-0987-5594
dc.identifier.scopus2-s2.0-84948845306
dc.identifier.urihttp://hdl.handle.net/11449/172255
dc.language.isoeng
dc.relation.ispartofScientific World Journal
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleLive and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7en
dc.typeArtigo
unesp.author.lattes0053567153623569[5]
unesp.author.lattes9304600166583100[4]
unesp.author.orcid0000-0002-1747-6158[5]
unesp.author.orcid0000-0003-0987-5594[4]

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Artigos - Microbiologia e Imunologia - IBB