Use of sorghum seed tissue as a biocatalyst in a stirred reactor for oxalic acid determination

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dc.contributor.authorFernandes, João Roberto [UNESP]
dc.contributor.authorDe Oliveira Neto, Graciliano
dc.contributor.authorKubota, Lauro T.
dc.contributor.authorTubino, Matthieu
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-27T11:18:07Z
dc.date.available2014-05-27T11:18:07Z
dc.date.issued1996-11-01
dc.description.abstractThe enzyme oxalate oxidase, E.C. 1.2.3.4 from Sorghum vulgare seeds (variety BR303) was used to develop a new sensor for oxalate determination without any purification. The sorghum seeds were conditioned in a 0.10 mol I-1 KCl solution. Then, these seeds were put in a stirring bar type enzymic reactor and coupled with an electrode for CO2. This device was introduced into a cell containing 10.0 ml of a 0.10 mol I-1 KCl solution saturated with oxygen. This sensor showed a linear response between 1.0 and 4.0 × 10-3 mol I-1 with a slope of 30 mV per decade of oxalate concentration at 25.0°C. The sensor was stable for one month or 200 determinations. The response time was about 60 s. The Michaelis-Menten constant determined for this enzyme was 1.5 × 10-3 mol I-1.en
dc.description.affiliationDepartamento de Química FC UNESP, PO Box 473, 17033-360, Bauru, SP
dc.description.affiliationInstituto de Química Unicamp, PO Box 6154, 13083-970 Campinas, SP
dc.description.affiliationUnespDepartamento de Química FC UNESP, PO Box 473, 17033-360, Bauru, SP
dc.format.extent397-399
dc.identifierhttp://dx.doi.org/10.1039/AC9963300397
dc.identifier.citationAnalytical Communications, v. 33, n. 11, p. 397-399, 1996.
dc.identifier.doi10.1039/AC9963300397
dc.identifier.issn1359-7337
dc.identifier.lattes8540599256820672
dc.identifier.scopus2-s2.0-0000678174
dc.identifier.urihttp://hdl.handle.net/11449/64871
dc.identifier.wosWOS:A1996VT08900003
dc.language.isoeng
dc.relation.ispartofAnalytical Communications
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.titleUse of sorghum seed tissue as a biocatalyst in a stirred reactor for oxalic acid determinationen
dc.typeArtigo
dcterms.licensehttp://www.rsc.org/AboutUs/Copyright/Authordeposition.asp
unesp.author.lattes8540599256820672
unesp.author.orcid0000-0001-8189-2618[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências, Baurupt
unesp.departmentQuímica - FCpt

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