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Haemagglutination induced by Bordetella pertussis filamentous haemagglutinin adhesin (FHA) is inhibited by antibodies produced against FHA(430-873) fragment expressed in Lactobacillus casei

dc.contributor.authorColombi, Debora
dc.contributor.authorOliveira, Maria L. S.
dc.contributor.authorCampos, Ivana B.
dc.contributor.authorMonedero, Vicente
dc.contributor.authorPerez-Martinez, Gaspar
dc.contributor.authorHo, Paulo L.
dc.contributor.institutionInstituto Butantan
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionCSIC
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:25:58Z
dc.date.available2014-05-20T15:25:58Z
dc.date.issued2006-12-01
dc.description.abstractFilamentous haemagglutinin adhesin (FHA) is an important virulence factor from Bordetella pertussis related to the adhesion and spread of the bacteria through the respiratory tract. Three distinct domains have been characterized in mature FHA, and among them, the FHA(442-863) fragment was suggested to be responsible for the heparin-binding activity. In this study, we cloned the gene encoding the HEP fragment (FHA(430-873)) in a Lactobacillus casei-inducible expression vector based on the lactose operon. The recombinant bacteria, transformed with the resulting construct (L. casei-HEP), were able to express the heterologous protein depending on the sugar added to the culture. Subcutaneous inoculation of L. casei-HEP in Balb/C mice, using the cholera toxin B subunit as adjuvant, induced systemic anti-HEP antibodies that were able to inhibit in vitro erythrocyte haemagglutination induced by FHA. This is the first example of a B. pertussis antigen produced in lactic acid bacteria and opens new perspectives for alternative vaccine strategies against whooping cough.en
dc.description.affiliationInst Butantan, Ctr Biotecnol, São Paulo, Brazil
dc.description.affiliationUSP, Inst Quim, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciências Biomed, Interunidades Biotecnol, BR-05508 São Paulo, Brazil
dc.description.affiliationCSIC, Inst Agroquim & Tecnol Alimentos, Burjassot, Valencia, Spain
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Botucatu
dc.format.extent462-466
dc.identifierhttp://dx.doi.org/10.1007/s00284-005-0388-0
dc.identifier.citationCurrent Microbiology. New York: Springer, v. 53, n. 6, p. 462-466, 2006.
dc.identifier.doi10.1007/s00284-005-0388-0
dc.identifier.issn0343-8651
dc.identifier.urihttp://hdl.handle.net/11449/36282
dc.identifier.wosWOS:000242360000002
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofCurrent Microbiology
dc.relation.ispartofjcr1.373
dc.relation.ispartofsjr0,562
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleHaemagglutination induced by Bordetella pertussis filamentous haemagglutinin adhesin (FHA) is inhibited by antibodies produced against FHA(430-873) fragment expressed in Lactobacillus caseien
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.orcid0000-0002-2586-1793[1]
unesp.author.orcid0000-0003-4849-3062[2]
unesp.author.orcid0000-0001-7461-8047[4]
unesp.author.orcid0000-0003-0334-0572[3]
unesp.author.orcid0000-0003-3501-1626[5]
unesp.author.orcid0000-0003-3652-241X[6]

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