Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland

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dc.contributor.authorSantos, Najara Akira Costa dos
dc.contributor.authorCarvalho, Vanessa Rafaela de [UNESP]
dc.contributor.authorSouza-Neto, Jayme A. [UNESP]
dc.contributor.authorAlonso, Diego Peres [UNESP]
dc.contributor.authorRibolla, Paulo Eduardo Martins [UNESP]
dc.contributor.authorMedeiros, Jansen Fernandes
dc.contributor.authorAraujo, Maisa da Silva
dc.contributor.institutionFundação Universidade Federal de Rondônia/Fiocruz Rondônia
dc.contributor.institutionFiocruz Rondônia
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionFundação Universidade Federal de Rondônia
dc.contributor.institutionCentro de Pesquisa em Medicina Tropical
dc.date.accessioned2023-07-29T16:14:57Z
dc.date.available2023-07-29T16:14:57Z
dc.date.issued2023-05-01
dc.description.abstractAnopheles darlingi is a major malaria vector in the Amazon region and, like other vectors, harbors a community of microorganisms with which it shares a network of interactions. Here, we describe the diversity and bacterial composition from the midguts and salivary glands of lab-reared and field-captured An. darlingi using metagenome sequencing of the 16S rRNA gene. The libraries were built using the amplification of the region V3–V4 16S rRNA gene. The bacterial community from the salivary glands was more diverse and richer than the community from the midguts. However, the salivary glands and midguts only showed dissimilarities in beta diversity between lab-reared mosquitoes. Despite that, intra-variability was observed in the samples. Acinetobacter and Pseudomonas were dominant in the tissues of lab-reared mosquitoes. Sequences of Wolbachia and Asaia were both found in the tissue of lab-reared mosquitoes; however, only Asaia was found in field-captured An. darlingi, but in low abundance. This is the first report on the characterization of microbiota composition from the salivary glands of An. darlingi from lab-reared and field-captured individuals. This study can provide invaluable insights for future investigations regarding mosquito development and interaction between mosquito microbiota and Plasmodium sp.en
dc.description.affiliationPrograma de Pós-Graduação em Biologia Experimental Departament of Medicine Fundação Universidade Federal de Rondônia/Fiocruz Rondônia, RO
dc.description.affiliationPlataforma de Produção e Infecção de Vetores da Malária-PIVEM Laboratório de Entomologia Fiocruz Rondônia, RO
dc.description.affiliationMultiuser Central Laboratory Department of Bioprocesses and Biotechnology School of Agricultural Sciences São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Biotecnology (IBTEC–Campus Botucatu) Instituto de Biotecnologia da UNESP Universidade Estadual Paulista (UNESP), SP
dc.description.affiliationPrograma de Pós-Graduação em Conservação e uso de Recursos Naturais–PPGReN Departament of Biology Fundação Universidade Federal de Rondônia, Campus José Ribeiro Filho, RO
dc.description.affiliationLaboratório de Pesquisa Translacional e Clínica Centro de Pesquisa em Medicina Tropical, RO
dc.description.affiliationUnespMultiuser Central Laboratory Department of Bioprocesses and Biotechnology School of Agricultural Sciences São Paulo State University (UNESP), SP
dc.description.affiliationUnespDepartment of Biotecnology (IBTEC–Campus Botucatu) Instituto de Biotecnologia da UNESP Universidade Estadual Paulista (UNESP), SP
dc.description.sponsorshipBill and Melinda Gates Foundation
dc.description.sponsorshipIdBill and Melinda Gates Foundation: INV-003970
dc.identifierhttp://dx.doi.org/10.3390/microorganisms11051145
dc.identifier.citationMicroorganisms, v. 11, n. 5, 2023.
dc.identifier.doi10.3390/microorganisms11051145
dc.identifier.issn2076-2607
dc.identifier.scopus2-s2.0-85160402189
dc.identifier.urihttp://hdl.handle.net/11449/249996
dc.language.isoeng
dc.relation.ispartofMicroorganisms
dc.sourceScopus
dc.subject16S rRNA
dc.subjectAmazon region
dc.subjectbacteria
dc.subjectmalaria vector
dc.subjectmicrobiota
dc.subjectmidgut
dc.subjectsalivary gland
dc.titleBacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Glanden
dc.typeArtigo
unesp.author.orcid0000-0002-2229-464X[2]
unesp.author.orcid0000-0003-4992-6253[4]
unesp.author.orcid0000-0001-8735-6090[5]

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