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Genetic diversity of ‘Candidatus Liberibacter asiaticus’ in Brazil analyzed in different geographic regions and citrus varieties

dc.contributor.authorde Paula, Larissa Bonevaes [UNESP]
dc.contributor.authorLin, Hong
dc.contributor.authorStuchi, Eduardo Sanches
dc.contributor.authorFrancisco, Carolina Sardinha
dc.contributor.authorSafady, Nágela Gomes
dc.contributor.authorColetta-Filho, Helvécio Della
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUSDA / San Joaquin Valley Agricultural Sciences Center
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionETH Zürich
dc.contributor.institutionInstituto Agronômico (IAC)/Centro de Citricultura Sylvio Moreira
dc.date.accessioned2019-10-06T15:33:51Z
dc.date.available2019-10-06T15:33:51Z
dc.date.issued2019-01-01
dc.description.abstractThe bacterium ‘Candidatus Liberibacter asiaticus’ - CLas, the agent associated with citrus huanglongbing disease was first reported in Sao Paulo State in 2004 and has spread throughout the citrus-growing regions of Sao Paulo and further to the states of Minas Gerais and Parana. However, little information is available regarding the genetic diversity of CLas since its introduction. Understanding the genetic diversity of this bacterium is important for tracing migration routes and for identifying evolutionary selection forces that may affect the genetic diversity of this pathogen. Total DNA from 199 HLB-diseased citrus species trees was sampled from geographic regions in Sao Paulo, Parana and Minas Gerais states and the CLas isolates were genotyped by simple sequence DNA repeats (SSR). Nei’s genetic diversity index was observed to be low in all populations (H Nei = 0.11–0.26). Wright’s fixation index (F ST ), which measures population genetic differentiation, did not differ significantly between CLas populations from Sao Paulo State and from Minas Gerais, but significant values (F ST = 0.118–0.191) of the Parana CLas population distinguish it from the others. Interestingly, higher values (F ST = 0.275–0.445) were observed for the CLas populations obtained from different citrus species compared to sweet orange, suggesting that the citrus genotypes could be driven the genetic diversity of CLas. Clustering analysis supports the F ST results that split the CLas samples into three genetically distinct populations. These results indicate that genetically homogeneous populations of CLas infect sweet orange plants in various regions of Sao Paulo State and Minas Gerais, but not Parana, suggesting that different introduction events may have occurred for the Sao Paulo and Parana states.en
dc.description.affiliationCampus de Jaboticabal Graduate Program in Genetics and Plant Breeding University of Estadual Paulista - UNESP
dc.description.affiliationUSDA / San Joaquin Valley Agricultural Sciences Center
dc.description.affiliationEstação Experimental de Citricultura de Bebedouro/EMBRAPA
dc.description.affiliationPlant Pathology Institute of Integrative Biology ETH Zürich
dc.description.affiliationInstituto Agronômico (IAC)/Centro de Citricultura Sylvio Moreira
dc.description.affiliationUnespCampus de Jaboticabal Graduate Program in Genetics and Plant Breeding University of Estadual Paulista - UNESP
dc.identifierhttp://dx.doi.org/10.1007/s10658-019-01695-1
dc.identifier.citationEuropean Journal of Plant Pathology.
dc.identifier.doi10.1007/s10658-019-01695-1
dc.identifier.issn1573-8469
dc.identifier.issn0929-1873
dc.identifier.scopus2-s2.0-85061667744
dc.identifier.urihttp://hdl.handle.net/11449/187365
dc.language.isoeng
dc.relation.ispartofEuropean Journal of Plant Pathology
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCitrus pathogen
dc.subjectGenetic diversity
dc.subjectGreening
dc.subjectSSR
dc.titleGenetic diversity of ‘Candidatus Liberibacter asiaticus’ in Brazil analyzed in different geographic regions and citrus varietiesen
dc.typeResenha

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