Cytogenotoxic evaluations of leaves and stems extracts of Rubus rosifolius in primary metabolically noncompetent cells

Página do item simplificado
dc.contributor.authorde Quadros, Ana Paula Oliveira [UNESP]
dc.contributor.authorBaraldi, Isabel Bragança [UNESP]
dc.contributor.authorPetreanu, Marcel
dc.contributor.authorNiero, Rivaldo
dc.contributor.authorMantovani, Mario Sergio
dc.contributor.authorDe Mascarenhas Gaivão, Isabel O’Neill
dc.contributor.authorMaistro, Edson Luis [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionVale do Itajaí University (UNIVALI)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversity of Trás-os-Montes and Alto Douro
dc.date.accessioned2023-07-29T16:12:04Z
dc.date.available2023-07-29T16:12:04Z
dc.date.issued2023-01-01
dc.description.abstractPlants with medicinal potential may also produce adverse effects in humans. This seems to be the case for the species Rubus rosifolius, where preliminary studies demonstrated genotoxic effects attributed to extracts obtained from leaves and stems of this plant using on HepG2/C3A human hepatoma cells as a model. Considering the beneficial properties of this plant as an antidiarrheal, analgesic, antimicrobial, and antihypertensive and its effects in the treatment of gastrointestinal diseases, the present study was developed with the aim of determining the cytotoxic and genotoxic potential of extracts of leaves and stems of R. rosifolius in primary without metabolic competence in human peripheral blood mononuclear cells (PBMC). Cell viability analyses at concentrations of between 0.01 and 100 µg/ml of both extracts did not markedly affect cell viability. In contrast, assessment of the genotoxic potential using the comet assay demonstrated significant damage to DNA within PBMC from a concentration of 10 µg/ml in the stem extract, and a clastogenic/aneugenic response without cytokinesis-block proliferation index (CBPI) alterations at concentrations of 10, 20, or 100 µg/ml for both extracts. Under our experimental conditions, the data obtained demonstrated genotoxic and mutagenic effects attributed to extracts from leaves and stems of R. rosifolius in cells in the absence of hepatic metabolism.en
dc.description.affiliationPost-Graduate Program on General and Applied Biology São Paulo State University UNESP – Biosciences Institute, SP
dc.description.affiliationSpeech and Hearing Therapy Department São Paulo State University – UNESP – Faculty of Philosophy and Sciences, SP
dc.description.affiliationDepartment of Biological Sciences Vale do Itajaí University (UNIVALI), SC
dc.description.affiliationLondrina State University–UEL–Department of General Biology, PR
dc.description.affiliationCECAV and Department of Genetics and Biotechnology University of Trás-os-Montes and Alto Douro
dc.description.affiliationUnespPost-Graduate Program on General and Applied Biology São Paulo State University UNESP – Biosciences Institute, SP
dc.description.affiliationUnespSpeech and Hearing Therapy Department São Paulo State University – UNESP – Faculty of Philosophy and Sciences, SP
dc.format.extent361-371
dc.identifierhttp://dx.doi.org/10.1080/15287394.2023.2203190
dc.identifier.citationJournal of Toxicology and Environmental Health - Part A: Current Issues, v. 86, n. 11, p. 361-371, 2023.
dc.identifier.doi10.1080/15287394.2023.2203190
dc.identifier.issn1087-2620
dc.identifier.issn1528-7394
dc.identifier.scopus2-s2.0-85153605301
dc.identifier.urihttp://hdl.handle.net/11449/249893
dc.language.isoeng
dc.relation.ispartofJournal of Toxicology and Environmental Health - Part A: Current Issues
dc.sourceScopus
dc.subjectcomet assay
dc.subjectgenotoxicity of Rubus rosifolius
dc.subjectmicronucleus test
dc.subjectmutagenicity of Rubus rosifolius
dc.subjectRosaceae
dc.titleCytogenotoxic evaluations of leaves and stems extracts of Rubus rosifolius in primary metabolically noncompetent cellsen
dc.typeArtigo
unesp.author.orcid0000-0001-5268-6508[5]

Arquivos

Coleções

Artigos - Fonoaudiologia - FFC