Field-deployable viral diagnostics using CRISPR-Cas13

dc.contributor.authorMyhrvold, Cameron
dc.contributor.authorFreije, Catherine A.
dc.contributor.authorGootenberg, Jonathan S.
dc.contributor.authorAbudayyeh, Omar O.
dc.contributor.authorMetsky, Hayden C.
dc.contributor.authorDurbin, Ann F.
dc.contributor.authorKellner, Max J.
dc.contributor.authorTan, Amanda L.
dc.contributor.authorPaul, Lauren M.
dc.contributor.authorParham, Leda A.
dc.contributor.authorGarcia, Kimberly F.
dc.contributor.authorBarnes, Kayla G.
dc.contributor.authorChak, Bridget
dc.contributor.authorMondini, Adriano [UNESP]
dc.contributor.authorNogueira, Mauricio L.
dc.contributor.authorIsern, Sharon
dc.contributor.authorMichael, Scott F.
dc.contributor.authorLorenzana, Ivette
dc.contributor.authorYozwiak, Nathan L.
dc.contributor.authorMacInnis, Bronwyn L.
dc.contributor.authorBosch, Irene
dc.contributor.authorGehrke, Lee
dc.contributor.authorZhang, Feng
dc.contributor.authorSabeti, Pardis C.
dc.contributor.institutionBroad Inst Massachusetts Inst Technol MIT & Harva
dc.contributor.institutionHarvard Univ
dc.contributor.institutionHarvard Med Sch
dc.contributor.institutionFlorida Gulf Coast Univ
dc.contributor.institutionUniv Nacl Autonoma Honduras
dc.contributor.institutionHarvard Sch Publ Hlth
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFac Med Sao Jose do Rio Preto
dc.contributor.institutionMt Sinai Sch Med
dc.description.abstractMitigating global infectious disease requires diagnostic tools that are sensitive, specific, and rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform can detect Zika virus (ZIKV) and dengue virus (DENV) in patient samples at concentrations as low as 1 copy per microliter. We developed HUDSON (heating unextracted diagnostic samples to obliterate nucleases), a protocol that pairs with SHERLOCK for viral detection directly from bodily fluids, enabling instrument-free DENVdetection directly from patient samples in <2 hours. We further demonstrate that SHERLOCK can distinguish the four DENV serotypes, as well as region-specific strains of ZIKV from the 2015-2016 pandemic. Finally, we report the rapid (<1 week) design and testing of instrument-free assays to detect clinically relevant viral single-nucleotide polymorphisms.en
dc.description.affiliationBroad Inst Massachusetts Inst Technol MIT & Harva, Cambridge, MA 02142 USA
dc.description.affiliationHarvard Univ, Dept Organismal & Evolutionary Biol, Ctr Syst Biol, Cambridge, MA 02138 USA
dc.description.affiliationHarvard Med Sch, Div Med Sci, PhD Program Virol, Boston, MA 02115 USA
dc.description.affiliationHarvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA
dc.description.affiliationMIT, McGovern Inst Brain Res, Cambridge, MA 02139 USA
dc.description.affiliationMIT, Dept Brain & Cognit Sci, Cambridge, MA 02139 USA
dc.description.affiliationMIT, Dept Biol Engn, Cambridge, MA 02139 USA
dc.description.affiliationMIT, Dept Hlth Sci & Technol, Cambridge, MA 02139 USA
dc.description.affiliationMIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA
dc.description.affiliationMIT, Inst Med Engn & Sci, Cambridge, MA 02139 USA
dc.description.affiliationFlorida Gulf Coast Univ, Dept Biol Sci, Ft Myers, FL 33965 USA
dc.description.affiliationUniv Nacl Autonoma Honduras, Inst Invest Microbiol, Ctr Invest Genet, Tegucigalpa, Honduras
dc.description.affiliationHarvard Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 USA
dc.description.affiliationSao Paulo State Univ, Sch Pharmaceut Sci, Araraquara Lab Publ Hlth, Sao Paulo, Brazil
dc.description.affiliationFac Med Sao Jose do Rio Preto, Lab Pesquisas Virol, Sao Paulo, Brazil
dc.description.affiliationMt Sinai Sch Med, Dept Med, New York, NY 10029 USA
dc.description.affiliationHarvard Med Sch, Dept Microbiol & Immunobiol, Boston, MA 02115 USA
dc.description.affiliationHHMI, Chevy Chase, MD 20815 USA
dc.description.affiliationUnespSao Paulo State Univ, Sch Pharmaceut Sci, Araraquara Lab Publ Hlth, Sao Paulo, Brazil
dc.description.sponsorshipBroad Institute Chemical Biology and Therapeutics Science Shark Tank
dc.description.sponsorshipDefense Advanced Research Projects Agency
dc.description.sponsorshipPaul and Daisy Soros fellowship
dc.description.sponsorshipNIH National Institute of Allergy and Infectious Diseases
dc.description.sponsorshipNew York Stem Cell foundation
dc.description.sponsorshipAllen foundation
dc.description.sponsorshipVallee foundation
dc.description.sponsorshipTan-Yang Center at MIT
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdNIH: U19AI110818
dc.description.sponsorshipIdNIH: F30 NRSA 1F30-CA210382
dc.description.sponsorshipIdNIH: AI 100190
dc.description.sponsorshipIdNIH: 1R01-HG009761
dc.description.sponsorshipIdNIH: 1R01-MH110049
dc.description.sponsorshipIdNIH: 1DP1-HL141201
dc.description.sponsorshipIdDefense Advanced Research Projects Agency: D18AC00006
dc.description.sponsorshipIdNIH National Institute of Allergy and Infectious Diseases: R01AI099210
dc.description.sponsorshipIdFAPESP: 13/21719-3
dc.identifier.citationScience. Washington: Amer Assoc Advancement Science, v. 360, n. 6387, p. 444-448, 2018.
dc.publisherAmer Assoc Advancement Science
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleField-deployable viral diagnostics using CRISPR-Cas13en
dcterms.rightsHolderAmer Assoc Advancement Science[14][14]
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