Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum

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dc.contributor.authorRossetto, Isabela Maria Urra
dc.contributor.authorCagnon, Valéria Helena Alves
dc.contributor.authorKido, Larissa Akemi
dc.contributor.authorLizarte Neto, Fermino Sanches
dc.contributor.authorTirapelli, Luís Fernando
dc.contributor.authorTirapelli, Daniela Pretti Da Cunha
dc.contributor.authorDe Almeida Chuffa, Luiz Gustavo [UNESP]
dc.contributor.authorMartinez, Francisco Eduardo [UNESP]
dc.contributor.authorMartinez, Marcelo
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2022-05-01T09:30:31Z
dc.date.available2022-05-01T09:30:31Z
dc.date.issued2021-08-01
dc.description.abstractCaffeine consumption is able to interfere in cellular processes related to inflammatory mechanisms by acting through the adenosinergic system. This study aimed to recognize alterations related to adenosinergic system and inflammatory process in the cerebellum of University of Chile Bibulous (UChB) rats after the consumption of ethanol and caffeine. UChB and Wistar rats, males at 5 months old, were divided into the groups (n = 15/group): (i) Control (Wistar rats receiving water); (ii) Ethanol group (UChB rats receiving ethanol solution at 10%) and (iii) Ethanol+caffeine group (UChB rats receiving ethanol solution at 10% added of 3 g/L of caffeine). The cerebellar tissue was collected and processed for immunohistochemistry, Reverse transcription polymerase chain reaction (RT-PCR) and western blotting techniques for the adenosinergic receptors A1 and A2a and inflammatory markers, including Nuclear factor kappa B (NFkB), TLR4, TLR2, MyD88, TNF-α, COX-2, iNOS and microglial marker Iba-1. Results showed ethanol and caffeine consumption differentially altering the immunolocalization of adenosinergic receptors and inflammatory markers in the cerebellar tissue. The A2a receptor was overexpressed in the Ethanol group and was evident in the glial cells. The Ethanol group had increased protein levels for NFκB and TLR4, expressively in Bergmann glia and Purkinje cells. Caffeine reduced the expression of these markers to levels similar to those found in the Control group. The A1 gene was upregulated the Ethanol group, but not its protein levels, suggesting post-Transcriptional interference. In conclusion, caffeine seems to attenuate ethanol-induced inflammation in the cerebellum of UChB rats through the A1 and A2a modulation, playing a neuroprotective role in the chronic context of ethanol consumption.en
dc.description.affiliationDepartment of Structural and Functional Biology University of Campinas (UNICAMP), 255 Monteiro Lobato St
dc.description.affiliationDepartment of Food and Nutrition University of Campinas (UNICAMP), 80 Monteiro Lobato St
dc.description.affiliationDepartment of Surgery and Anatomy University of São Paulo (USP), 3900 Bandeirantes Ave
dc.description.affiliationDepartment of Structural and Functional Biology State University of São Paulo (UNESP), 250 Prof. Dr. Antônio Celso Wagner Zanin St
dc.description.affiliationDepartment of Morphology and Pathology Federal University of São Carlos (UFSCar), 13571 Biblioteca Comunitária Ave
dc.description.affiliationUnespDepartment of Structural and Functional Biology State University of São Paulo (UNESP), 250 Prof. Dr. Antônio Celso Wagner Zanin St
dc.format.extent835-849
dc.identifierhttp://dx.doi.org/10.1093/toxres/tfab067
dc.identifier.citationToxicology Research, v. 10, n. 4, p. 835-849, 2021.
dc.identifier.doi10.1093/toxres/tfab067
dc.identifier.issn2045-4538
dc.identifier.issn2045-452X
dc.identifier.scopus2-s2.0-85115016020
dc.identifier.urihttp://hdl.handle.net/11449/233544
dc.language.isoeng
dc.relation.ispartofToxicology Research
dc.sourceScopus
dc.subjectadenosinergic receptor
dc.subjectcaffeine
dc.subjectcerebellum
dc.subjectethanol
dc.subjectinflammation
dc.subjectUChB rats
dc.titleCaffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellumen
dc.typeArtigo

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