Combination of extractive techniques followed by HPLC-MS/MS analysis to monitor ent-agathic acid in fish treated with Copaifera duckei Dwyer

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dc.contributor.authorda Silva, Jonas Joaquim Mangabeira
dc.contributor.authorCampanharo, Sarah Chagas
dc.contributor.authorda Silva, Agnaldo Fernando Baldo
dc.contributor.authorde Jesus, Raphael Barbetta [UNESP]
dc.contributor.authorFigueredo, Tais Alexandra Mário
dc.contributor.authorPilarski, Fabiana [UNESP]
dc.contributor.authorHeleno, Vladimir Constantino Gomes
dc.contributor.authorPaschoal, Jonas Augusto Rizzato
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity de Franca (UNIFRAN)
dc.date.accessioned2023-07-29T13:56:34Z
dc.date.available2023-07-29T13:56:34Z
dc.date.issued2023-05-30
dc.description.abstractPlants are used as therapeutic alternatives in Veterinary Medicine, including therapies for food-producing animals. However, these medicinal resources can sometimes contain dangerous substances, and when used in animals that supply food, they stand out from the point of view of food safety. The diterpene ent-agathic acid, a component of Copaifera duckei oleoresin, is an example of substances already described with toxic activity in mammals. Thus, this study aimed to propose combining two extractive techniques followed by high-performance liquid chromatography coupled mass spectrometry analysis to monitor residues of ent-agathic acid in Piaractus mesopotamicus fillet treated in an immersion bath with Copaifera duckei oleoresin. An optimized combination of solid–liquid extraction (using acidified acetonitrile) and dispersive liquid–liquid microextraction (using acidified water and chloroform as dispersive and extracting solvent, respectively) was performed to recover the target analyte, added to the development of HPLC-MS/MS method with adequate validation parameters to quantify the ent-agathic acid present in the fish fillet. In vivo tests of residual persistence of ent-agathic acid in fishes treated with C. duckei oleoresin were performed, indicating the non-detection of the target diterpene (< 6.1 µg/mL). The combined extractive procedure followed by quantitative analysis in the in vivo test of residual persistence of the target analyte in fish indicated the absence of ent-agathic acid in all samples. Thus, the data found might contribute to understanding the use of oleoresins from C. duckei as an alternative to traditional veterinary products.en
dc.description.affiliationSchool of Pharmaceutical Sciences of Ribeirão Preto Department of Biomolecular Science – University of São Paulo (FCFRP-USP)
dc.description.affiliationFaculty of Philosophy Sciences and Letters at Ribeirão Preto Department of Chemistry – University of São Paulo (FCFRP-USP)
dc.description.affiliationLaboratory of Microbiology and Parasitology of Aquatic Organisms (LAPOA) Aquaculture Center (CAUNESP) – São Paulo State University (UNESP)
dc.description.affiliationNucleus of Research in Sciences and Technology University de Franca (UNIFRAN)
dc.description.affiliationUnespLaboratory of Microbiology and Parasitology of Aquatic Organisms (LAPOA) Aquaculture Center (CAUNESP) – São Paulo State University (UNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012/18334-0
dc.description.sponsorshipIdFAPESP: 2017/15052-7
dc.description.sponsorshipIdFAPESP: 2019/06159-8
dc.identifierhttp://dx.doi.org/10.1016/j.jchromb.2023.123763
dc.identifier.citationJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, v. 1224.
dc.identifier.doi10.1016/j.jchromb.2023.123763
dc.identifier.issn1873-376X
dc.identifier.issn1570-0232
dc.identifier.scopus2-s2.0-85160355782
dc.identifier.urihttp://hdl.handle.net/11449/248893
dc.language.isoeng
dc.relation.ispartofJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
dc.sourceScopus
dc.subjectAquaculture
dc.subjectDLLME
dc.subjectExtraction
dc.subjectFood safety
dc.subjectLC-MS/MS
dc.subjectToxic residue
dc.titleCombination of extractive techniques followed by HPLC-MS/MS analysis to monitor ent-agathic acid in fish treated with Copaifera duckei Dwyeren
dc.typeArtigo

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