Enzymatic synthesis of capric acid-rich structured lipids and their effects on mice with high-fat diet-induced obesity

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dc.contributor.authorMartínez-Galán, Julián Paul
dc.contributor.authorOntibón-Echeverri, Catalina María
dc.contributor.authorCampos Costa, Mariana [UNESP]
dc.contributor.authorBatista-Duharte, Alexander [UNESP]
dc.contributor.authorGuerso Batista, Vinicius [UNESP]
dc.contributor.authorMesa, Victoria
dc.contributor.authorMonti, Rubens [UNESP]
dc.contributor.authorVeloso de Paula, Ariela [UNESP]
dc.contributor.authorMartins Baviera, Amanda [UNESP]
dc.contributor.institutionUniversity of Antioquia (UdeA)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:30:45Z
dc.date.available2022-04-29T08:30:45Z
dc.date.issued2021-10-01
dc.description.abstractThe objective of this study was to produce structured lipids (SLs) by enzymatic acidolysis using Rhizopus oryzae lipase covalently immobilized in a low-cost material. Grape seed oil was used to synthesize SLs containing the medium-chain fatty acid (C10:0) capric acid. SL synthesis led to 38.8% medium-chain fatty acid incorporation with 5 reuses of the enzymatic derivative. The reaction conditions for the synthesis of MLM-TAGs (triacylglycerols with one long- and two medium-chain acyl residues) were at a molar ratio of fatty acid:oil of 3:1, performed at 40 °C and lipase immobilized load of 5% (w/w). The in vivo effects of SLs were studied in Swiss mice fed premade diets: control (C) diet, high-fat diet (HFD) with 100% lipid content as lard, HFD with 50% lipid content as grape seed oil (HG) or HFD with 50% lipid content as capric acid-containing SLs produced from grape seed oil (HG-MCT). Mice from HG and HG-MCT groups had decreases in body weight gain and reductions in the weights of white adipose tissues. In addition, HG and HG-MCT mice had low plasma levels of glucose and total cholesterol, and improvements in the glucose tolerance. HG and HG-MCT diets have remarkable antioxidant properties, since low plasma levels of TBARS (thiobarbituric acid reactive substances, biomarkers of lipid peroxidation) were found in mice fed these diets. Interestingly, TBARS levels in HG-MCT mice were further decreased than values of HG mice. Mice fed HG and HG-MCT diets also showed preservation in the activity of the antioxidant enzyme paraoxonase 1. Both HG and HG-MCT diets promoted reduction of IL-6 and IL-10 production by splenocytes. The capric acid-containing SLs produced from grape seed oil emerges as a functional oil capable to mitigate obesity complications resulting from oxidative stress and inflammation.en
dc.description.affiliationSchool of Nutrition and Dietetic University of Antioquia (UdeA), Calle 70 No. 52-21
dc.description.affiliationSchool of Pharmaceutical Sciences Department of Clinical Analysis São Paulo State University (Unesp)
dc.description.affiliationSchool of Pharmaceutical Sciences Department of Bioprocess Engineering and Biotechnology São Paulo State University (Unesp)
dc.description.affiliationUnespSchool of Pharmaceutical Sciences Department of Clinical Analysis São Paulo State University (Unesp)
dc.description.affiliationUnespSchool of Pharmaceutical Sciences Department of Bioprocess Engineering and Biotechnology São Paulo State University (Unesp)
dc.identifierhttp://dx.doi.org/10.1016/j.foodres.2021.110602
dc.identifier.citationFood Research International, v. 148.
dc.identifier.doi10.1016/j.foodres.2021.110602
dc.identifier.issn1873-7145
dc.identifier.issn0963-9969
dc.identifier.scopus2-s2.0-85110456549
dc.identifier.urihttp://hdl.handle.net/11449/229154
dc.language.isoeng
dc.relation.ispartofFood Research International
dc.sourceScopus
dc.subjectEnzymatic acidolysis
dc.subjectGlucose intolerance
dc.subjectInflammation
dc.subjectLipase
dc.subjectObesity
dc.subjectOxidative stress
dc.subjectStructured lipids
dc.titleEnzymatic synthesis of capric acid-rich structured lipids and their effects on mice with high-fat diet-induced obesityen
dc.typeArtigo
unesp.author.orcid0000-0002-1875-0518[4]
unesp.author.orcid0000-0003-2615-7033[6]
unesp.departmentAnálises Clínicas - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas