Staphylococcus epidermidis and Staphylococcus haemolyticus: detection of biofilm genes and biofilm formation in blood culture isolates from patients in a Brazilian teaching hospital

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dc.contributor.authorPinheiro, Luiza [UNESP]
dc.contributor.authorBrito, Carla Ivo [UNESP]
dc.contributor.authorOliveira, Adilson de [UNESP]
dc.contributor.authorPereira, Valéria Cataneli [UNESP]
dc.contributor.authorCunha, Maria de Lourdes Ribeiro de Souza da [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto Lauro de Souza Lima
dc.date.accessioned2018-12-11T17:06:16Z
dc.date.available2018-12-11T17:06:16Z
dc.date.issued2016-09-01
dc.description.abstractInfections with coagulase-negative staphylococci are often related to biofilm formation. This study aimed to detect biofilm formation and biofilm-associated genes in blood culture isolates of Staphylococcus epidermidis and S. haemolyticus. Half (50.6%) of the 85 S. epidermidis isolates carried the icaAD genes and 15.3% the bhp gene, while these numbers were 42.9% and 0 for S. haemolyticus, respectively. According to the plate test, 30 S. epidermidis isolates were biofilm producers and 40% of them were strongly adherent, while only one (6%) of the 17 S. haemolyticus biofilm-producing isolates exhibited a strongly adherent biofilm. The concomitant presence of icaA and icaD was significantly associated with the plate and tube test results (P ≤ 0.0004). The higher frequency of icaA in S. epidermidis and of icaD in S. haemolyticus is correlated with the higher biofilm-producing capacity of the former since, in contrast to IcaD, IcaA activity is sufficient to produce small amounts of polysaccharide. Although this study emphasizes the importance of icaAD and bhp for biofilm formation in S. epidermidis, other mechanisms seem to be involved in S. haemolyticus.en
dc.description.affiliationDepartamento de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista UNESP
dc.description.affiliationLaboratório de Patologia Instituto Lauro de Souza Lima
dc.description.affiliationUnespDepartamento de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista UNESP
dc.format.extent11-14
dc.identifierhttp://dx.doi.org/10.1016/j.diagmicrobio.2016.06.006
dc.identifier.citationDiagnostic Microbiology and Infectious Disease, v. 86, n. 1, p. 11-14, 2016.
dc.identifier.doi10.1016/j.diagmicrobio.2016.06.006
dc.identifier.file2-s2.0-84989921728.pdf
dc.identifier.issn1879-0070
dc.identifier.issn0732-8893
dc.identifier.lattes0115647772315973
dc.identifier.scopus2-s2.0-84989921728
dc.identifier.urihttp://hdl.handle.net/11449/173557
dc.language.isoeng
dc.relation.ispartofDiagnostic Microbiology and Infectious Disease
dc.relation.ispartofsjr1,157
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectbhp
dc.subjectBiofilm
dc.subjectica genes
dc.subjectStaphylococcus epidermidis
dc.subjectStaphylococcus haemolyticus
dc.titleStaphylococcus epidermidis and Staphylococcus haemolyticus: detection of biofilm genes and biofilm formation in blood culture isolates from patients in a Brazilian teaching hospitalen
dc.typeArtigo
unesp.author.lattes0115647772315973

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Artigos - Microbiologia e Imunologia - IBB