Parents with periodontitis drive the early acquisition of dysbiotic microbiomes in their offspring

dc.contributor.authorReis, Aurélio Amorim
dc.contributor.authorMonteiro, Mabelle Freitas
dc.contributor.authorBonilha, Gabriela Martin
dc.contributor.authorSaraiva, Luciana
dc.contributor.authorAraújo, Cassia [UNESP]
dc.contributor.authorSantamaria, Mauro Pedrine [UNESP]
dc.contributor.authorCasati, Marcio Zaffalon
dc.contributor.authorKumar, Purnima
dc.contributor.authorCasarin, Renato Corrêa Viana
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Michigan
dc.description.abstractAim: To evaluate the microbial colonization in different dentition phases on individuals from 0 to 18 years of age belonging to families with a history of periodontitis compared to descendants of periodontally healthy parents. Materials and Methods: The offspring of subjects with periodontitis (‘Perio’ group) and the offspring of periodontally healthy subjects (‘Healthy’ group), matched for gender and age, were included in this cross-sectional study and divided according to the dentition phase: pre-dentate, primary, mixed and permanent. The patients were clinically assessed, and their saliva was collected. DNA was extracted, and V1–V3 and V4–V5 regions of the 16S rRNA gene were sequenced. Results: Fifty children of parents with periodontitis and 50 from healthy parents were included in the study and divided according to the dentition phase: pre-dentate (n = 5/group), primary dentition (n = 15/group), mixed dentition (n = 15/group) and permanent dentition (n = 15/group) in each group. The microbiome composition was different between dentitions for both groups. Children of the Perio group presented a microbial diversity different from that of the Healthy group in mixed and permanent dentitions. The more intense shift in the community occurred between primary and mixed dentition in the Perio group, while the transition between mixed and permanent dentition was the period with greater changes in the microbiome for the Healthy group. Furthermore, a pathogen-rich environment—higher prevalence and abundance of periodontitis-associated species such as Prevotella spp., Selenomonas spp., Leptotrichia spp., Filifactor alocis, Prevotella intermedia, Treponema denticola and Tannerella forsythia— was observed in the Perio group. Conclusions: The parents' periodontal status significantly affects the microbiome composition of their offspring from an early age. The mixed dentition was the phase associated with establishing a dysbiotic and pathogen-rich microbiome in descendants of parents with periodontitis.en
dc.description.affiliationPiracicaba Dental School State University of Campinas
dc.description.affiliationSchool of Dentistry University of São Paulo
dc.description.affiliationInstitute of Health Science São Paulo State University
dc.description.affiliationSchool of Dentistry University of Michigan
dc.description.affiliationUnespInstitute of Health Science São Paulo State University
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCNPq: 309429/2017-0
dc.description.sponsorshipIdCAPES: CAPES-001
dc.identifier.citationJournal of Clinical Periodontology, v. 50, n. 7, p. 890-904, 2023.
dc.relation.ispartofJournal of Clinical Periodontology
dc.subjectgrade C periodontitis
dc.subjectteeth eruption
dc.subjectvertical transmission
dc.titleParents with periodontitis drive the early acquisition of dysbiotic microbiomes in their offspringen