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Effects of scrotal insulation in Nellore bulls (Bos taurus indicus) on seminal quality and its relationship with in vitro fertilizing ability

dc.contributor.authorFernandes, C. E.
dc.contributor.authorDode, M. A. N.
dc.contributor.authorPereira, D.
dc.contributor.authorSilva, A. E. D. F. [UNESP]
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:19:09Z
dc.date.available2014-05-20T13:19:09Z
dc.date.issued2008-12-01
dc.description.abstractThe objectives of this study were to assess the effects of induced testicular degeneration in Bos taurus indicus (Nellore) bulls on changes in seminal characteristics and fertilizing ability of sperm. Four Nellore bulls (30-36-month-old, 500-550 kg) with good seminal quality (> 80% motile and morphologically normal sperm) had serotal insulation applied for 5 d. Semen was collected by electroejaculation and cryopreserved at a the pre-insulation moment, and 7, 14, and 21 d after insulation was removed. Gross motility, vigor of sperm movement (1-5), acrosome integrity, sperm morphology (phase-contrast microscopy), nuclear vacuoles and abnormal chromatin (Feulgen-stain) were determined after sperm preparations for in vitro fertilization (IVF). Prior to IVF, sperm were separated using a Percoll gradient (45% and 90%). Normal sperm decreased (P < 0.05) 14 and 21 d after insulation was removed. on 14 and 21 d, the incidence of head defects (9.7 +/- 0.6 and 17.0 +/- 0.8, respectively; mean +/- S.E.M) was higher (P < 0.05) in agreement with the incidence of nuclear vauoles (14.0 +/- 5.0 and 12.3 +/- 2.3) and abnormal chromatin (24.4 +/- 7.2 and 30.8 +/- 2.8). Although the frequency of cleaved oocytes decreased only on 21 d (P < 0.05), blastocyst rates were lower (P < 0.05) than pre-insulation on 14 and 21 d. In regression analyses, only nuclear vacuoles, head defects and intact acrosome accounted for differences in cleavage (R(2) = 0.38, 0.48, and 0.30, respectively) and blastocyst rates (R(2) = 0.35, 0.37, and 0.44). Abnormal chromatin was associated only with blastocyst rates (R(2) = 0.35). In conclusion, blastocyst rate was more sensitive than cleavage rate and the assessment of nuclear integrity is recommended to predict the fertilizing ability of bull sperm. (c) 2008 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de Mato Grosso do Sul (UFMS), Dept Patol, CCBS, BR-79070000 Campo Grande, MS, Brazil
dc.description.affiliationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Recursos Genet & Biotecnol, BR-70770990 Brasilia, DF, Brazil
dc.description.affiliationUNESP, Fac Med Vet & Zootecnia, BR-18618000 Jaboticabal, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Med Vet & Zootecnia, BR-18618000 Jaboticabal, SP, Brazil
dc.format.extent1560-1568
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2008.07.005
dc.identifier.citationTheriogenology. New York: Elsevier B.V., v. 70, n. 9, p. 1560-1568, 2008.
dc.identifier.doi10.1016/j.theriogenelogy.2008.07.005
dc.identifier.issn0093-691X
dc.identifier.lattes0423131924105070
dc.identifier.urihttp://hdl.handle.net/11449/4929
dc.identifier.wosWOS:000261285000020
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBovine semenen
dc.subjectSperm morphologyen
dc.subjectFertilizationen
dc.subjectAbnormal sperm chromatinen
dc.titleEffects of scrotal insulation in Nellore bulls (Bos taurus indicus) on seminal quality and its relationship with in vitro fertilizing abilityen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes0423131924105070
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentZootecnia - FCAVpt

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