Novel bovine-associated pVAPN plasmid type in Rhodococcus equi identified from lymph nodes of slaughtered cattle and lungs of people living with HIV/AIDS

dc.contributor.authorRibeiro, M. G. [UNESP]
dc.contributor.authorLara, G. H.B. [UNESP]
dc.contributor.authorda Silva, P.
dc.contributor.authorFranco, M. M.J. [UNESP]
dc.contributor.authorde Mattos-Guaraldi, A. L.
dc.contributor.authorde Vargas, A. P.C.
dc.contributor.authorSakate, R. I.
dc.contributor.authorPavan, F. R. [UNESP]
dc.contributor.authorColhado, B. S. [UNESP]
dc.contributor.authorPortilho, F. V.R. [UNESP]
dc.contributor.authorMotta, R. G.
dc.contributor.authorKakuda, T.
dc.contributor.authorTakai, S.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionIAL-Adolfo Lutz Institute
dc.contributor.institutionUniversidade do Estado do Rio de Janeiro (UERJ)
dc.contributor.institutionUniversidade Federal de Sergipe (UFS)
dc.contributor.institutionFederal Veterinary Inspection Service
dc.contributor.institutionUniRV-University of Rio Verde
dc.contributor.institutionKitasato University
dc.date.accessioned2018-12-11T17:17:13Z
dc.date.available2018-12-11T17:17:13Z
dc.date.issued2018-04-01
dc.description.abstractRhodococcus equi is a well-recognized Gram-positive intracellular facultative bacterium that is opportunistic in nature, which causes pyogranulomatous infections in humans and multiple host animals. The pathogenicity of the microorganism has been attributed to the presence of plasmid-encoded virulence-associated proteins (Vap). To date, three host-associated virulence plasmid types of R. equi have been identified as follows: the circular pVAPA and pVAPB, related, respectively, to equine and porcine isolates, and a recently described linear pVAPN plasmid associated with bovine strains, although these three types are found in human isolates. Recent phylogenomic studies support the evidence that human R. equi infection is zoonotically acquired. Nevertheless, data regarding distribution and prevalence of the host-adapted virulence plasmid types of R. equi isolated from meat animals are scarce or unnoticed. Here, the three host-associated virulence plasmid types (pVAPA, pVAPB, and pVAPN) were investigated in 154 R. equi isolates recovered from lymph nodes of cattle with lymphadenitis (n = 31), faeces of cattle without enteric signs (n = 49), as well as different clinical specimens from human patients (n = 74). The analysis of virulence profile of 74 R. equi from humans revealed six (8.1%) isolates pVAPB (type 8), two (2.7%) pVAPN, and one (1.3%) pVAPB (type 11), all of which were from lung samples from people living with HIV/AIDS. From the lymph node samples of cattle, 41.9% (13 of 31) isolates revealed pVAPN type, whereas all isolates from faecal samples were negative for three host-associated types. Here, recently described bovine-associated pVAPN type was detected in R. equi isolates recovered from the lungs of people living with HIV/AIDS and lymph nodes from slaughtered cattle intended for human consumption; a finding that represents a public health concern, mainly in countries where undercooked or raw meat are traditionally consumed.en
dc.description.affiliationDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP-São Paulo State University
dc.description.affiliationIAL-Adolfo Lutz Institute
dc.description.affiliationUERJ-Rio de Janeiro State University
dc.description.affiliationUFSM-Federal University of Santa Maria
dc.description.affiliationFederal Veterinary Inspection Service
dc.description.affiliationUNESP-Faculty of Pharmacy
dc.description.affiliationUniRV-University of Rio Verde
dc.description.affiliationKitasato University
dc.description.affiliationUnespDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP-São Paulo State University
dc.description.affiliationUnespUNESP-Faculty of Pharmacy
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/20585-9
dc.format.extent321-326
dc.identifierhttp://dx.doi.org/10.1111/tbed.12785
dc.identifier.citationTransboundary and Emerging Diseases, v. 65, n. 2, p. 321-326, 2018.
dc.identifier.doi10.1111/tbed.12785
dc.identifier.issn1865-1682
dc.identifier.issn1865-1674
dc.identifier.scopus2-s2.0-85040010798
dc.identifier.urihttp://hdl.handle.net/11449/175716
dc.language.isoeng
dc.relation.ispartofTransboundary and Emerging Diseases
dc.relation.ispartofsjr1,147
dc.relation.ispartofsjr1,147
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectbovine rhodococcosis
dc.subjectemerging disease
dc.subjecthost-associated plasmid types
dc.subjectimmunocompromised human patients
dc.subjectpVAPN
dc.titleNovel bovine-associated pVAPN plasmid type in Rhodococcus equi identified from lymph nodes of slaughtered cattle and lungs of people living with HIV/AIDSen
dc.typeArtigo
unesp.author.orcid0000-0003-2682-9389[1]

Arquivos