Effect of different periods of preconditioning with saliva on adhesion of C. albicans to a denture base resin by crystal violet staining and XTT assay.

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dc.contributor.authorZamperini, Camila Andrade
dc.contributor.authorSchiavinato, Patrícia Cristiane dos Santos
dc.contributor.authorMachado, Ana Lucia [UNESP]
dc.contributor.authorGiampaolo, Eunice Teresinha
dc.contributor.authorVergani, Carlos Eduardo [UNESP]
dc.contributor.authorPavarina, Ana Cláudia
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-08-06T16:13:07Z
dc.date.available2015-08-06T16:13:07Z
dc.date.issued2010
dc.description.abstractAim: The role of saliva on Candida adhesion to biomaterials has not been clearly defined. The present study investigates whether different periods of preconditioning with saliva would influence the adhesion of Candida albicans to a denture base resin. Methods: Ninety samples of acrylic resin with smooth surfaces were made and then divided into five groups: one control without saliva, and four experimental groups conditioned in saliva for periods of 30 min, 1, 3, or 12 h. Candida adhesion was evaluated by crystal violet staining and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-([phenylamino] carbonyl)-2H-tetrazolium-hydroxide assay. Results: The one-way analysis of variance revealed that there were no significant differences among the mean number of adherent cells or among the mean absorbance for all groups. No significant correlation was found between the two methods used for assessing Candida albicans adhesion. Conclusion: The different periods of preconditioning with saliva had no significant influence on the adhesion of Candida albicans to the denture base acrylic resin.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Araraquara, Faculdade de Odontologia de Araraquara Unesp, Araraquara, Rua Humaitá, nº 1680 - Departamento de Materiais Odontológicos e Prótese, Centro, CEP 14801-903, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Araraquara, Faculdade de Odontologia de Araraquara Unesp, Araraquara, Rua Humaitá, nº 1680 - Departamento de Materiais Odontológicos e Prótese, Centro, CEP 14801-903, SP, Brasil
dc.format.extent114-119
dc.identifier.citationJournal of Investigative and Clinical Dentistry, v. 1, n. 2, p. 114-119, 2010.
dc.identifier.doi10.1111/j.2041-1626.2010.00013.x
dc.identifier.issn2041-1626
dc.identifier.lattes5737127334248549
dc.identifier.lattes8867670539105403
dc.identifier.lattes8000248781842587
dc.identifier.lattes3003130522427820
dc.identifier.lattes7298585988482083
dc.identifier.orcid0000-0002-7375-4714
dc.identifier.urihttp://hdl.handle.net/11449/125789
dc.language.isoeng
dc.relation.ispartofJournal of Investigative and Clinical Dentistry
dc.relation.ispartofsjr0,433
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectacrylic resinen
dc.subjectadhesionen
dc.subjectbiofilmen
dc.subjectCandida albicansen
dc.subjectsalivaen
dc.titleEffect of different periods of preconditioning with saliva on adhesion of C. albicans to a denture base resin by crystal violet staining and XTT assay.en
dc.typeArtigo
unesp.author.lattes5737127334248549(4)
unesp.author.lattes8000248781842587[3]
unesp.author.lattes3003130522427820[5]
unesp.author.lattes7298585988482083
unesp.author.lattes8867670539105403[6]
unesp.author.orcid0000-0002-7375-4714[5]
unesp.author.orcid0000-0002-9231-1994[6]
unesp.author.orcid0000-0002-2718-428X[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt
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