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Phenylalanine ammonia-lyase activity in soybean roots extract measured by reverse-phase high performance liquid chromatography

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dc.contributor.authorFerrarese, MLL
dc.contributor.authorRodrigues, J. D.
dc.contributor.authorFerrarese, O.
dc.contributor.institutionUniv Maringa
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:48:17Z
dc.date.available2014-05-20T13:48:17Z
dc.date.issued2000-03-01
dc.description.abstractThe high performance liquid chromatography (HPLC) technique was applied to measure phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activity in soybean (Glycine max L. Merril cv. BR16) roots. t-Cinnamate, the catalytic product of the PAL reaction was quantified at 275 nm by isocratic elution with methanol:water through an ODS(M) column. Comparative experiments were carried out with 1.0 mM ferulic acid, an inducer of PAL activity. The results suggest that liquid chromatography is a rapid and sensitive method to analyze PAL activity in non-purified extract.en
dc.description.affiliationUniv Maringa, Dept Biochem, BR-87020900 Maringa, Parana, Brazil
dc.description.affiliationUNESP, IB, Dept Bot, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, IB, Dept Bot, BR-18618000 Botucatu, SP, Brazil
dc.format.extent152-153
dc.identifierhttp://dx.doi.org/10.1055/s-2000-9162
dc.identifier.citationPlant Biology. Stuttgart: Georg Thieme Verlag, v. 2, n. 2, p. 152-153, 2000.
dc.identifier.doi10.1055/s-2000-9162
dc.identifier.issn1435-8603
dc.identifier.lattes4211432128816409
dc.identifier.urihttp://hdl.handle.net/11449/17212
dc.identifier.wosWOS:000086431400004
dc.language.isoeng
dc.publisherGeorg Thieme Verlag
dc.relation.ispartofPlant Biology
dc.relation.ispartofjcr2.156
dc.relation.ispartofsjr0,939
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectt-cinnamatept
dc.subjectHPLCpt
dc.subjectPALpt
dc.subjectsoybeanpt
dc.titlePhenylalanine ammonia-lyase activity in soybean roots extract measured by reverse-phase high performance liquid chromatographyen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderGeorg Thieme Verlag
dspace.entity.typePublication
unesp.author.lattes4211432128816409
unesp.author.orcid0000-0001-7965-4696[2]
unesp.author.orcid0000-0001-5312-614X[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.departmentBotânica - IBBpt

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Botucatu - IBB - Instituto de Biociências