Transcriptomic insight into the hybridization mechanism of the Tambacu, a hybrid from Colossoma macropomum (Tambaqui) and Piaractus mesopotamicus (Pacu)

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dc.contributor.authorMareco, Edson Assunção
dc.contributor.authorde la Serrana, Daniel Garcia
dc.contributor.authorde Paula, Tassiana Gutierrez [UNESP]
dc.contributor.authorZanella, Bruna Tereza Thomazini [UNESP]
dc.contributor.authorda Silva Duran, Bruno Oliveira [UNESP]
dc.contributor.authorSalomão, Rondinelle Artur Simões
dc.contributor.authorde Almeida Fantinatti, Bruno Evaristo [UNESP]
dc.contributor.authorde Oliveira, Victor Hugo Garcia
dc.contributor.authordos Santos, Vander Bruno
dc.contributor.authorCarvalho, Robson Francisco [UNESP]
dc.contributor.authorDal-Pai-Silva, Maeli [UNESP]
dc.contributor.institutionUniversity of Western São Paulo
dc.contributor.institutionUniversity of Barcelona
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionInstituto de Pesca/APTA/SAA
dc.date.accessioned2023-07-29T15:15:13Z
dc.date.available2023-07-29T15:15:13Z
dc.date.issued2023-03-01
dc.description.abstractInterspecific hybrids are highly complex organisms, especially considering aspects related to the organization of genetic material. The diversity of possibilities created by the genetic combination between different species makes it difficult to establish a large-scale analysis methodology. An example of this complexity is Tambacu, an interspecific hybrid of Colossoma macropomum (Tambaqui) and Piaractus mesopotamicus (Pacu). Either genotype represents an essential role in South American aquaculture. However, despite this importance, the genetic information for these genotypes is still highly scarce in specialized databases. Using RNA-Seq analysis, we characterized the transcriptome of white muscle from Pacu, Tambaqui, and their interspecific hybrid (Tambacu). The sequencing process allowed us to obtain a significant number of reads (approximately 53 billion short reads). A total of annotated contigs were 37,285, 96,738, and 158,709 for Pacu, Tambaqui, and Tambacu. After that, we performed a comparative analysis of the transcriptome of the three genotypes, where we evaluated the differential expression (Tambacu vs Pacu = 11,156, and Tambacu vs Tambaqui = 876) profile of the transcript and the degree of similarity between the nucleotide sequences between the genotypes. We assessed the intensity and pattern of expression across genotypes using differential expression information. Clusterization analysis showed a closer relationship between Tambaqui and Tambacu. Furthermore, digital differential expression analysis selected some target genes related to essential cellular processes to evaluate and validate the expression through the RT-qPCR. The RT-qPCR analysis demonstrated significantly (p < 0.05) elevated expression of the mafbx, foxo1a, and rgcc genes in the hybrid compared to the parents. Likewise, we can observe genes significantly more expressed in Pacu (mtco1 and mylpfa) and mtco2 in Tambaqui. Our results showed that the phenotype presented by Tambacu might be associated with changes in the gene expression profile and not necessarily with an increase in gene variability. Thus, the molecular mechanisms underlying these “hybrid effects” may be related to additive and, in some cases, dominant regulatory interactions between parental alleles that act directly on gene regulation in the hybrid transcripts.en
dc.description.affiliationEnvironment and Regional Development Graduate Program University of Western São Paulo, São Paulo
dc.description.affiliationCell Biology Physiology and Immunology Department School of Biology University of Barcelona, Catalonia
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Bioscience of Botucatu São Paulo State University, São Paulo
dc.description.affiliationInstituto de Pesca/APTA/SAA
dc.description.affiliationBiology Department University of Western São Paulo, São Paulo
dc.description.affiliationUnespDepartment of Structural and Functional Biology Institute of Bioscience of Botucatu São Paulo State University, São Paulo
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2016/05009-4.
dc.description.sponsorshipIdCNPq: 432602/2018-6
dc.identifierhttp://dx.doi.org/10.1016/j.cbd.2022.101041
dc.identifier.citationComparative Biochemistry and Physiology - Part D: Genomics and Proteomics, v. 45.
dc.identifier.doi10.1016/j.cbd.2022.101041
dc.identifier.issn1878-0407
dc.identifier.issn1744-117X
dc.identifier.scopus2-s2.0-85142682996
dc.identifier.urihttp://hdl.handle.net/11449/249407
dc.language.isoeng
dc.relation.ispartofComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
dc.sourceScopus
dc.subjectAquaculture
dc.subjectmRNA expression
dc.subjectMuscle growth
dc.subjectRNA-Seq
dc.subjectSkeletal muscle
dc.subjectTeleost fish
dc.titleTranscriptomic insight into the hybridization mechanism of the Tambacu, a hybrid from Colossoma macropomum (Tambaqui) and Piaractus mesopotamicus (Pacu)en
dc.typeArtigo

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