Cloning of glucocorticoid-regulated genes in C6/ST1 rat glioma phenotypic reversion

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dc.contributor.authorValentini, Sandro Roberto [UNESP]
dc.contributor.authorArmelin, M. C. S.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:18:03Z
dc.date.available2014-05-27T11:18:03Z
dc.date.issued1996-01-01
dc.description.abstractThe C6 rat glioma cell line is responsive to glucocorticoid hormones. C6 variants that are hyper-responsive (ST1) and resistant (P7) to hormone treatment have been derived previously. Glucocorticoid treatment of ST1 cells leads to complete reversion of the transformed phenotype and loss of tumorigenic potential. Production of C type retrovirus particles is also induced by glucocorticoids in ST1 cells. Cloning of the genes regulated by glucocorticoids in this cell system was used here as a strategy to uncover the gene products involved in the transformed-to-normal phenotypic change. Construction of a cDNA library from glucocorticoid-treated ST1 cells and screening by differential hybridization resulted in the isolation of three cellular sequences that code for rat metallothioneins (C27 and C41) and α1-acid glycoprotein (C36). Northern blot analysis revealed that expression of these genes was dramatically induced by hydrocortisone in ST1 but not in P7 cells. Viral genomic RNA was used to isolate and characterize retrovirus-related sequences that could also be responsible for the phenotypic reversion phenomenon.en
dc.description.affiliationInstituto de Química Universidade de São Paulo Departamento de Bioquímica, CP 26.077, São Paulo, 05599-970, SP
dc.description.affiliationFaculdade de Cie. Farmaceuticas Universidade Estadual Paulista Depto. de Cie. Biológicas, CP 502, Araraquara, 14801-902, SP
dc.description.affiliationUnespFaculdade de Cie. Farmaceuticas Universidade Estadual Paulista Depto. de Cie. Biológicas, CP 502, Araraquara, 14801-902, SP
dc.format.extent11-17
dc.identifierhttp://dx.doi.org/10.1677/joe.0.1480011
dc.identifier.citationJournal of Endocrinology, v. 148, n. 1, p. 11-17, 1996.
dc.identifier.doi10.1677/joe.0.1480011
dc.identifier.issn0022-0795
dc.identifier.lattes5333250355049814
dc.identifier.scopus2-s2.0-0030029574
dc.identifier.urihttp://hdl.handle.net/11449/64716
dc.identifier.wosWOS:A1996TM75400002
dc.language.isoeng
dc.relation.ispartofJournal of Endocrinology
dc.relation.ispartofjcr4.012
dc.relation.ispartofsjr1,993
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectcycloheximide
dc.subjecthydrocortisone
dc.subjectanimal cell
dc.subjectcell cloning
dc.subjectcontrolled study
dc.subjectcytology
dc.subjectgene expression
dc.subjectglioma cell
dc.subjectmolecular biology
dc.subjectnonhuman
dc.subjectphenotype
dc.subjectpriority journal
dc.subjectrat
dc.subjectAnimals
dc.subjectBlotting, Northern
dc.subjectCell Line, Transformed
dc.subjectCloning, Molecular
dc.subjectGlioma
dc.subjectHydrocortisone
dc.subjectIn Situ Hybridization
dc.subjectMetallothionein
dc.subjectOrosomucoid
dc.subjectPhenotype
dc.subjectRats
dc.subjectRetroviridae
dc.subjectRNA
dc.subjectTumor Cells, Cultured
dc.subjectViral Envelope Proteins
dc.subjectVirus Activation
dc.titleCloning of glucocorticoid-regulated genes in C6/ST1 rat glioma phenotypic reversionen
dc.typeArtigo
unesp.author.lattes5333250355049814
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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