Colitis induced by dextran sulphate sodium causes histopathological and immunological changes in the periodontal tissues of Wistar rats

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dc.contributor.authorde Mello-Neto, João Martins
dc.contributor.authorElangovan, Gayathiri
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorJohnson, Newell Walter
dc.contributor.authorGustafsson, Anders
dc.contributor.authorda Figueredo, Carlos Marcelo
dc.contributor.institutionGriffith University
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionKarolinska Institutet
dc.date.accessioned2023-07-29T14:52:37Z
dc.date.available2023-07-29T14:52:37Z
dc.date.issued2022-12-01
dc.description.abstractObjective: This study investigated the impact of colitis induced by dextran sulphate sodium (DSS)-induced colitis (DIC) on histopathological and immunological outcomes in the periodontal tissues of Wistar rats. Background: Inflammatory bowel diseases (IBD) and periodontitis have been reported to present a bidirectional relationship. However, the inflammatory pathway that connects both diseases needs further investigation. Material and Methods: Twenty-five male Wistar rats were allocated in four groups: unilateral ligature-induced periodontitis for 14 days: LIP (n = 7); dextran sulphate sodium-induced colitis only: DIC (n = 6); DIC + LIP (n = 6) and controls (n = 6). Digital images were obtained from the histological sections. In order to assess the attachment loss (AL), the linear distance between the cementoenamel junction (CEJ) and the alveolar bone crest was measured on the mesial root using histological photomicrography's ImageJ software. Immunological analyses of gingival tissues and plasma were performed by Bio-Plex Th1/Th2 Assay. Results: The DIC group showed inflammatory cells extending to the periodontal connective tissues, which contained significantly elevated expressions of IL-1α, IL-1β, IL-2, IL-6, IL-12, IL-13, GM-CSF, IFN-γ and TNF-α compared to controls. There was no significant difference in bone loss between controls and DIC. There were no significant histopathological differences between DIC + LIP and LIP. However, DIC + LIP presented a significantly lower IL-2 and IL-5 than the LIP group. There was no bone loss difference between LIP+DIC and LIP groups. DIC + LIP group presented significantly higher levels of GM-CSF in plasma. Conclusion: DSS-induced colitis was associated with an overexpression of Th1/Th2- related cytokines in the gingival tissue.en
dc.description.affiliationSchool of Medicine and Dentistry Griffith University
dc.description.affiliationDepartment of Basic Sciences School of Dentistry São Paulo State University-UNESP
dc.description.affiliationDivision of Oral Diseases Department of Dental Medicine Karolinska Institutet
dc.description.affiliationUnespDepartment of Basic Sciences School of Dentistry São Paulo State University-UNESP
dc.description.sponsorshipAustralian Dental Research Foundation
dc.format.extent1267-1276
dc.identifierhttp://dx.doi.org/10.1111/jre.13063
dc.identifier.citationJournal of Periodontal Research, v. 57, n. 6, p. 1267-1276, 2022.
dc.identifier.doi10.1111/jre.13063
dc.identifier.issn1600-0765
dc.identifier.issn0022-3484
dc.identifier.scopus2-s2.0-85139949781
dc.identifier.urihttp://hdl.handle.net/11449/249270
dc.language.isoeng
dc.relation.ispartofJournal of Periodontal Research
dc.sourceScopus
dc.subjectcytokines
dc.subjectdextran sulphate sodium
dc.subjectgingiva
dc.subjectinflammatory bowel diseases
dc.subjectperiodontitis
dc.subjectrats
dc.titleColitis induced by dextran sulphate sodium causes histopathological and immunological changes in the periodontal tissues of Wistar ratsen
dc.typeArtigo

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