How adaptive laboratory evolution can boost yeast tolerance to lignocellulosic hydrolyses

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dc.contributor.authorMenegon, Yasmine Alves [UNESP]
dc.contributor.authorGross, Jeferson [UNESP]
dc.contributor.authorJacobus, Ana Paula [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T19:52:51Z
dc.date.available2022-04-28T19:52:51Z
dc.date.issued2022-01-01
dc.description.abstractThe yeast Saccharomyces cerevisiae is an excellent candidate for establishing cell factories to convert lignocellulosic biomass into chemicals and fuels. To enable this technology, yeast robustness must be improved to withstand the fermentation inhibitors (e.g., weak organic acids, phenols, and furan aldehydes) resulting from biomass pretreatment and hydrolysis. Here, we discuss how evolution experiments performed in the lab, a method commonly known as adaptive laboratory evolution (ALE), may contribute to lifting yeast tolerance against the inhibitors of lignocellulosic hydrolysates (LCHs). The key is that, through the combination of whole-genome sequencing and reverse engineering, ALE provides a robust platform for discovering and testing adaptive alleles, allowing to explore the genetic underpinnings of yeast responses to LCHs. We review the insights gained from past evolution experiments with S. cerevisiae in LCH inhibitors and propose experimental designs to optimise the discovery of genetic variants adaptive to biomass toxicity. The knowledge gathered through ALE projects is envisaged as a roadmap to engineer superior yeast strains for biomass-based bioprocesses.en
dc.description.affiliationInstitute for Bioenergy Research São Paulo State University (UNESP), São Paulo
dc.description.affiliationUnespInstitute for Bioenergy Research São Paulo State University (UNESP), São Paulo
dc.identifierhttp://dx.doi.org/10.1007/s00294-022-01237-z
dc.identifier.citationCurrent Genetics.
dc.identifier.doi10.1007/s00294-022-01237-z
dc.identifier.issn1432-0983
dc.identifier.issn0172-8083
dc.identifier.scopus2-s2.0-85127426242
dc.identifier.urihttp://hdl.handle.net/11449/223749
dc.language.isoeng
dc.relation.ispartofCurrent Genetics
dc.sourceScopus
dc.subjectAdaptive laboratory evolution
dc.subjectLignocellulosic hydrolysates
dc.subjectMolecular genetics
dc.subjectSaccharomyces cerevisiae
dc.subjectSecond-generation ethanol
dc.titleHow adaptive laboratory evolution can boost yeast tolerance to lignocellulosic hydrolysesen
dc.typeResenha
unesp.author.orcid0000-0002-0871-7300[1]
unesp.author.orcid0000-0002-4537-2946[2]
unesp.author.orcid0000-0002-9092-931X[3]

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