Friedelin Synthase from Maytenus ilicifolia: Leucine 482 Plays an Essential Role in the Production of the Most Rearranged Pentacyclic Triterpene
dc.contributor.author | Souza-Moreira, Tatiana M. [UNESP] | |
dc.contributor.author | Alves, Thaís B. [UNESP] | |
dc.contributor.author | Pinheiro, Karina A. [UNESP] | |
dc.contributor.author | Felippe, Lidiane G. [UNESP] | |
dc.contributor.author | De Lima, Gustavo M. A. | |
dc.contributor.author | Watanabe, Tatiana F. [UNESP] | |
dc.contributor.author | Barbosa, Cristina C. [UNESP] | |
dc.contributor.author | Santos, Vânia A.F.F.M. [UNESP] | |
dc.contributor.author | Lopes, Norberto P. | |
dc.contributor.author | Valentini, Sandro R. [UNESP] | |
dc.contributor.author | Guido, Rafael V. C. | |
dc.contributor.author | Furlan, Maysa [UNESP] | |
dc.contributor.author | Zanelli, Cleslei F. [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidade de Saõ Paulo | |
dc.date.accessioned | 2018-12-11T17:23:17Z | |
dc.date.available | 2018-12-11T17:23:17Z | |
dc.date.issued | 2016-11-22 | |
dc.description.abstract | Among the biologically active triterpenes, friedelin has the most-rearranged structure produced by the oxidosqualene cyclases and is the only one containing a cetonic group. In this study, we cloned and functionally characterized friedelin synthase and one cycloartenol synthase from Maytenus ilicifolia (Celastraceae). The complete coding sequences of these 2 genes were cloned from leaf mRNA, and their functions were characterized by heterologous expression in yeast. The cycloartenol synthase sequence is very similar to other known OSCs of this type (approximately 80% identity), although the M. ilicifolia friedelin synthase amino acid sequence is more related to β-amyrin synthases (65-74% identity), which is similar to the friedelin synthase cloned from Kalanchoe daigremontiana. Multiple sequence alignments demonstrated the presence of a leucine residue two positions upstream of the friedelin synthase Asp-Cys-Thr-Ala-Glu (DCTAE) active site motif, while the vast majority of OSCs identified so far have a valine or isoleucine residue at the same position. The substitution of the leucine residue with valine, threonine or isoleucine in M. ilicifolia friedelin synthase interfered with substrate recognition and lead to the production of different pentacyclic triterpenes. Hence, our data indicate a key role for the leucine residue in the structure and function of this oxidosqualene cyclase. | en |
dc.description.affiliation | Instituto de Química Univ. Estadual Paulista UNESP, Rua Prof. Francisco Degni 55 | |
dc.description.affiliation | Centro de Pesquisa e Inovaçaõ em Biodiversidade e Fármacos Instituto de Física de Saõ Carlos Universidade de Saõ Paulo | |
dc.description.affiliation | Faculdade de Ciências Farmaceûticas Univ. Estadual Paulista-UNESP, Rod. Araraquara-Jaú km 1 | |
dc.description.affiliation | Faculdade de Ciências Farmaceûticas Universidade de Saõ Paulo, Avenida do Café s/n | |
dc.description.affiliationUnesp | Instituto de Química Univ. Estadual Paulista UNESP, Rua Prof. Francisco Degni 55 | |
dc.description.affiliationUnesp | Faculdade de Ciências Farmaceûticas Univ. Estadual Paulista-UNESP, Rod. Araraquara-Jaú km 1 | |
dc.identifier | http://dx.doi.org/10.1038/srep36858 | |
dc.identifier.citation | Scientific Reports, v. 6. | |
dc.identifier.doi | 10.1038/srep36858 | |
dc.identifier.file | 2-s2.0-84996940074.pdf | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.lattes | 1308042794786872 | |
dc.identifier.lattes | 1525665408900195 | |
dc.identifier.orcid | 0000-0001-7831-1149 | |
dc.identifier.scopus | 2-s2.0-84996940074 | |
dc.identifier.uri | http://hdl.handle.net/11449/176962 | |
dc.language.iso | eng | |
dc.relation.ispartof | Scientific Reports | |
dc.relation.ispartofsjr | 1,533 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Scopus | |
dc.title | Friedelin Synthase from Maytenus ilicifolia: Leucine 482 Plays an Essential Role in the Production of the Most Rearranged Pentacyclic Triterpene | en |
dc.type | Artigo | |
unesp.author.lattes | 1308042794786872 | |
unesp.author.lattes | 1525665408900195[13] | |
unesp.author.orcid | 0000-0001-7831-1149[13] | |
unesp.department | Fármacos e Medicamentos - FCF | pt |
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