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Gene expression of paracoccidioides virulence factors after interaction with macrophages and fibroblasts

dc.contributor.authorBraz, Jaqueline Derissi [UNESP]
dc.contributor.authorSardi, Janaina de Cássia Orlandi [UNESP]
dc.contributor.authorPitangui, Nayla de Souza [UNESP]
dc.contributor.authorVoltan, Aline Raquel [UNESP]
dc.contributor.authorAlmeida, Ana Marisa Fusco [UNESP]
dc.contributor.authorGiannini, Maria José Soares Mendes [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFederal University of Mato Grosso do Sul
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2021-06-25T10:57:26Z
dc.date.available2021-06-25T10:57:26Z
dc.date.issued2021-01-01
dc.description.abstractParacoccidioidomycosis (PCM) is a systemic mycosis with high prevalence in Latin America that is caused by thermodimorphic fungal species of the Paracoccidioides genus. In this study, we used quantitative PCR (qPCR) to investigate the expression of genes related to the virulence of Paracoccidioides brasiliensis (Pb18) and P. lutzii (Pb01) strains in their mycelial (M) and yeast (Y) forms after contact with alveolar macrophages (AMJ2-C11 cell line) and fibroblasts (MRC-5 cell line). The selected genes were those coding for 43 kDa glycoprotein (gp43), enolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 14-3-3 protein (30 kDa), phospholipase, and aspartyl protease. In the Pb18 M form, the aspartyl protease gene showed the highest expression among all genes tested, both before and after infection of host cells. In the Pb18 Y form after macrophage infection, the 14-3-3 gene showed the highest expression among all genes tested, followed by the phospholipase and gp43 genes, and their expression was 50-fold, 10-fold, and 6-fold higher, respectively, than that in the M form. After fibroblast infection with the Pb18 Y form, the 14-3-3 gene showed the highest expression, followed by the phospholipase and aspartyl protease genes, and their expression was 25-fold, 10-fold, and 10-fold higher, respectively, than that in the M form. Enolase and aspartyl protease genes were expressed upon infection of both cell lines. After macrophage infection with the Pb01 Y form, the 14-3-3 gene showed the highest expression, followed by the phospholipase and aspartyl protease genes, and their expression was 18-fold, 12.5-fold, and 6-fold higher, respectively, than that in the M form. In conclusion, the data show that the expression of the genes analyzed may be upregulated upon fungus-host interaction. Therefore, these genes may be involved in the pathogenesis of paracoccidioidomycosis.en
dc.description.affiliationDepartment of Clinical Analysis Laboratory of Clinical Mycology Faculty of Pharmaceutical Sciences UNESP – Univ Estadual Paulista
dc.description.affiliationSchool of Pharmaceutical Sciences Food and Nutrition Federal University of Mato Grosso do Sul
dc.description.affiliationDepartment of Cellular and Molecular Biology Ribeirão Preto School of Medicine University of São Paulo
dc.description.affiliationUnespDepartment of Clinical Analysis Laboratory of Clinical Mycology Faculty of Pharmaceutical Sciences UNESP – Univ Estadual Paulista
dc.format.extent1-24
dc.identifierhttp://dx.doi.org/10.1590/0074-02760200592
dc.identifier.citationMemorias do Instituto Oswaldo Cruz, v. 116, n. 1, p. 1-24, 2021.
dc.identifier.doi10.1590/0074-02760200592
dc.identifier.fileS0074-02762021000100306.pdf
dc.identifier.issn1678-8060
dc.identifier.issn0074-0276
dc.identifier.scieloS0074-02762021000100306
dc.identifier.scopus2-s2.0-85103745027
dc.identifier.urihttp://hdl.handle.net/11449/207568
dc.language.isoeng
dc.relation.ispartofMemorias do Instituto Oswaldo Cruz
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAdhesin genes
dc.subjectM form
dc.subjectParacoccidioides spp
dc.subjectVirulence factors
dc.subjectY form
dc.titleGene expression of paracoccidioides virulence factors after interaction with macrophages and fibroblastsen
dc.typeArtigo
unesp.departmentAnálises Clínicas - FCFpt

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