Genotoxic effects of mercury chloride on the neotropical fish andinoacara rivulatus (Cichlidae: Cichlasomatini)

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dc.contributor.authorNirchio, Mauro
dc.contributor.authorChoco-Veintimilla, Oscar
dc.contributor.authorQuizhpe-Cordero, Patricio Fredy
dc.contributor.authorHernández, José Gregorio
dc.contributor.authorOliveira, Claudio [UNESP]
dc.contributor.institutionFacultad de Ciencias Agropecuarias
dc.contributor.institutionNúcleo Nueva Esparta
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T19:28:06Z
dc.date.available2022-04-28T19:28:06Z
dc.date.issued2019-09-01
dc.description.abstractUnderstanding the effects of heavy metals in aquatic ecosystems is of significant importance due to their potential to bioaccumulate at various trophic levels and induce damage in DNA. Mercury is considered one of the most dangerous heavy metals, causing chromosomal breakage (clastogenic event) or spindle dysfunction (aneugenic event), that can lead to the formation of encapsulated chromatin into a separate smaller nucleus, generally referred to as a micronucleus. We evaluated the sensitivity of the micronucleus test in the neotropical cichlid Andinoacara rivulatus (Günther 1860). The fish were divided into four groups of 16 individuals, and each group was placed in separate aquaria (140 L) provided with filtered water and constant aeration. Fish were exposed to mercury chloride (HgCl2) at doses 0.1, 0.25, and 0.50 mg/kg body weight, administered by intraperitoneal (IP) injection. Fish from the control group were injected with a physiologic solution. The following erythrocyte anomalies were identified: erythrocytes with micronuclei varying to some extent in size and position in the cytoplasm, blebbed nucleus, binucleated cell, nuclei showing a deep invagination toward the center (notched nuclei). Examination of blood smears demonstrated a higher level of micronucleus and notched erythrocytes in fish injected with HgCl2 than in the controls. There were significant differences in the frequency of micronucleated and notched erythrocytes among the groups exposed to mercury. Linear regression analysis revealed a positive relationship between the frequency of micronucleated and notched erythrocytes (P< 0.0001), with a moderately strong correlation coefficient (R= 0.737). We propose that, in addition to the two so far known mechanisms of micronucleus formation (spindle apparatus damage and chromosomal ruptures), chromatin fragmentation in notched nuclei resulting from a combination of the cytotoxic effects of mercury and mechanical stress, may be a third mechanism of micronuclei genesis.en
dc.description.affiliationUniversidad Técnica de Machala Facultad de Ciencias Agropecuarias, Av. Panamericana Km. 5 1/2 Vía a Pasaje
dc.description.affiliationUniversidad de Oriente Núcleo Nueva Esparta
dc.description.affiliationDepartamento de Morfologia Instituto de Biociências Universidade Estadual Paulista
dc.description.affiliationUnespDepartamento de Morfologia Instituto de Biociências Universidade Estadual Paulista
dc.format.extent745-754
dc.identifierhttp://dx.doi.org/10.15517/rbt.v67i4.34133
dc.identifier.citationRevista de Biologia Tropical, v. 67, n. 4, p. 745-754, 2019.
dc.identifier.doi10.15517/rbt.v67i4.34133
dc.identifier.issn2215-2075
dc.identifier.issn0034-7744
dc.identifier.scopus2-s2.0-85074475334
dc.identifier.urihttp://hdl.handle.net/11449/221392
dc.language.isoeng
dc.relation.ispartofRevista de Biologia Tropical
dc.sourceScopus
dc.subjectDNA damage
dc.subjectErythrocyte nuclear abnormalities
dc.subjectFish
dc.subjectHeavy metal
dc.subjectmicronuclei
dc.titleGenotoxic effects of mercury chloride on the neotropical fish andinoacara rivulatus (Cichlidae: Cichlasomatini)en
dc.titleEfectos genotóxicos del cloruro de mercurio en el pez neotropical andinoacara rivulatus (Cichlidae: cichlasomatini).es
dc.typeArtigo

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