Cryptosporidium proventriculi in Captive Cockatiels (Nymphicus hollandicus)

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dc.contributor.authorPanegossi, Mariele Fernanda da Cruz [UNESP]
dc.contributor.authorWidmer, Giovanni
dc.contributor.authorNagata, Walter Bertequini
dc.contributor.authorOliveira, Bruno César Miranda
dc.contributor.authorFerrari, Elis Domingos
dc.contributor.authorGomes, Jancarlo Ferreira
dc.contributor.authorMeireles, Marcelo Vasconcelos [UNESP]
dc.contributor.authorNakamura, Alex Akira [UNESP]
dc.contributor.authordo Santos-Doni, Thaís Rabelo
dc.contributor.authorda Silveira Neto, Luiz
dc.contributor.authorBresciani, Katia Denise Saraiva [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionTufts University
dc.contributor.institutionSecretaria de Agricultura e Abastecimento do Estado de São Paulo
dc.contributor.institutionUnião das Faculdades dos Grandes Lagos (Unilago)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de Viçosa (UFV)
dc.contributor.institutionUniversidade Federal do Tocantins (UFT)
dc.date.accessioned2023-07-29T16:14:48Z
dc.date.available2023-07-29T16:14:48Z
dc.date.issued2023-05-01
dc.description.abstractCockatiels (Nymphicus hollandicus) are among the most commonly sold psittacines pets. The aim of this study was to evaluate the occurrence of Cryptosporidium spp. in domestic N. hollandicus and identify risk factors for this infection. We collected fecal samples from 100 domestic cockatiels in the city of Araçatuba, São Paulo, Brazil. Feces from birds of both genders and older than two months were collected. Owners were asked to complete a questionnaire to identify how they handle and care for their birds. Based on nested PCR targeting the 18S rRNA gene, the prevalence of Cryptosporidium spp. in the cockatiels sampled was 9.00%, 6.00% based on Malachite green staining, 5.00% based on modified Kinyoun straining, and 7.00% when the Malachite green was combined with Kinyoun. Applying multivariate logistic regression to test the association between Cryptosporidium proventriculi positivity and potential predictors showed that gastrointestinal alterations was a significant predictor (p < 0.01). Amplicons from five samples were sequenced successfully and showed 100% similarity with C. proventriculi. In summary, this study demonstrates the occurrence of C. proventriculi in captive cockatiels.en
dc.description.affiliationFaculdade de Medicina Veterinária Universidade Estadual Paulista (Unesp), São Paulo
dc.description.affiliationDepartment of Infectious Disease & Global Health Cummings School of Veterinary Medicine Tufts University
dc.description.affiliationEscritório de Defesa Agropecuária Coordenadoria de Defesa Agropecuária Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Lins, São Paulo
dc.description.affiliationUnião das Faculdades dos Grandes Lagos (Unilago), São Paulo
dc.description.affiliationFaculdade de Ciências Médicas e Instituto de Computação Universidade Estadual de Campinas (UNICAMP), São Paulo
dc.description.affiliationInstituto de Ciências Agrárias (ICA) Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Avenida Universitários, Minas Gerais
dc.description.affiliationImunologia e Vacinologia curso de Engenharia de Bioprocessos e Biotecnologia Universidade Federal do Tocantins (UFT), Tocantins
dc.description.affiliationUnespFaculdade de Medicina Veterinária Universidade Estadual Paulista (Unesp), São Paulo
dc.identifierhttp://dx.doi.org/10.3390/pathogens12050710
dc.identifier.citationPathogens, v. 12, n. 5, 2023.
dc.identifier.doi10.3390/pathogens12050710
dc.identifier.issn2076-0817
dc.identifier.scopus2-s2.0-85160352232
dc.identifier.urihttp://hdl.handle.net/11449/249993
dc.language.isoeng
dc.relation.ispartofPathogens
dc.sourceScopus
dc.subject18S rRNA gene
dc.subjectbirds
dc.subjectcryptosporidiosis
dc.subjectepidemiology
dc.subjectmolecular characterization
dc.subjectprevalence
dc.subjectrisk factors
dc.titleCryptosporidium proventriculi in Captive Cockatiels (Nymphicus hollandicus)en
dc.typeArtigo
unesp.author.orcid0000-0001-7255-3761[2]
unesp.author.orcid0000-0002-5444-6695[3]
unesp.author.orcid0000-0002-5114-3234[8]
unesp.author.orcid0000-0002-9279-9571[10]

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Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA
Artigos - Apoio, Produção e Saúde Animal - FMVA