Effect of radiotherapy on the differentiation and osteogenic activity of mesenchymal stem cells on dental implants

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dc.contributor.authorda COSTA, Fernanda Herrera [UNESP]
dc.contributor.authorDutra, Mateus José [UNESP]
dc.contributor.authorde VASCONCELLOS, Luana Marotta Reis [UNESP]
dc.contributor.authorVegian, Mariana Raquel da Cruz [UNESP]
dc.contributor.authorda SILVA, Camila Duarte [UNESP]
dc.contributor.authorDos Santos, Hanna Flávia Santana [UNESP]
dc.contributor.authorFederico, Claudio Antonio
dc.contributor.authorDo Prado, Renata Falchete [UNESP]
dc.contributor.authorTango, Rubens Nisie [UNESP]
dc.contributor.authorKaminagakura, Estela [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionInstituto de Estudos Avançados
dc.date.accessioned2023-07-29T13:37:42Z
dc.date.available2023-07-29T13:37:42Z
dc.date.issued2023-01-01
dc.description.abstractObjective: to evaluate the differentiation and gene expression of transcripts related to osteogenesis in a primary culture of Mesenchymal Stem Cells (MSCs) derived from rat femurs submitted to radiotherapy and the installation of pure titanium implants. Material and Methods: fifty-four rats received titanium implants in both femurs and were divided into three groups: Control: implant surgery (C); Implant + immediate irradiation (IrI), and Implant + late irradiation (IrL). Euthanasia occurred 3, 14, and 49 days after surgery. The bone marrow MSCs from the femurs were isolated and cultivated. The cell viability, total protein content, alkaline phosphatase (ALP) activity, and the formation of mineralization nodules and cellular genotoxicity were analyzed. The gene expression of Alkaline Phosphatase (phoA), Collagen 1 (COL1), Runt-related transcription factor 2 (RUNX2), Osterix (OSX), Osteopontin (OPN), Integrin β1 (ITGB1 ), Bone Sialoprotein (BSP), Osteonectin (SPARC), Osteocalcin (Bglap), Transforming Growth Factor β-type (TGF-β), Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF), Interleukin-6 (IL-6), Apolipoprotein E (APOE) and Prostaglandin E2 synthase (PGE2 ) were evaluated by qRT-PCR. Results: ionizing radiation suppresses the gene expression of essential transcripts for bone regeneration, as well as cellular viability, as observed in the IrI and IrL groups. Conclusion: although this can lead to the loss of osseointegration and failure of the implant, the MSCs showed more activity at 49 days than at 3 and 14 days.en
dc.description.affiliationUniversidade Estadual Paulista Instituto de Ciência e Tecnologia Departamento de Biociências e Diagnóstico Bucal, São Paulo
dc.description.affiliationInstituto de Estudos Avançados Departamento de Ciência e Tecnologia Aeroespacial, São Paulo
dc.description.affiliationUniversidade Estadual Paulista Instituto de Ciência e Tecnologia Departamento de Materiais Dentários e Prótese Dentária, São Paulo
dc.description.affiliationUnespUniversidade Estadual Paulista Instituto de Ciência e Tecnologia Departamento de Biociências e Diagnóstico Bucal, São Paulo
dc.description.affiliationUnespUniversidade Estadual Paulista Instituto de Ciência e Tecnologia Departamento de Materiais Dentários e Prótese Dentária, São Paulo
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 16/20103-7
dc.identifierhttp://dx.doi.org/10.4322/bds.2023.e3660
dc.identifier.citationBrazilian Dental Science, v. 26, n. 1, 2023.
dc.identifier.doi10.4322/bds.2023.e3660
dc.identifier.issn2178-6011
dc.identifier.scopus2-s2.0-85146382699
dc.identifier.urihttp://hdl.handle.net/11449/248214
dc.language.isoeng
dc.relation.ispartofBrazilian Dental Science
dc.sourceScopus
dc.subjectBone regeneration
dc.subjectCancer treatment protocol
dc.subjectDental implants
dc.subjectOsseointegration
dc.subjectOsteogenesis
dc.titleEffect of radiotherapy on the differentiation and osteogenic activity of mesenchymal stem cells on dental implantsen
dc.titleEfeito da radioterapia na diferenciação e atividade osteogênica de células-tronco mesenquimais em implantes dentáriospt
dc.typeArtigo

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Artigos - Biociências e Diagnóstico Bucal - ICT
Artigos - Materiais Odontológicos e Prótese - ICT