Supplementation with an Inorganic Zinc Source in the Metalloproteomic Profile of Royal Jelly in Apis mellifera L.

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dc.contributor.authorde Almeida Longuini, Aimê [UNESP]
dc.contributor.authorMartineli, Gabriel Moreno [UNESP]
dc.contributor.authorCamilli, Marcelo Polizel [UNESP]
dc.contributor.authorde Barros, Daniel Cavalcante Brambila [UNESP]
dc.contributor.authorVieira, José Cavalcante Souza [UNESP]
dc.contributor.authorde Magalhães Padilha, Pedro [UNESP]
dc.contributor.authorde Oliveira Orsi, Ricardo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T10:19:40Z
dc.date.available2021-06-25T10:19:40Z
dc.date.issued2021-01-01
dc.description.abstractThis study aimed to evaluate the quality of royal jelly produced by honeybees Apis mellifera supplemented with different concentrations of inorganic zinc (zinc sulfate monohydrate—0, 25, 50, and 75 ppm). Two-dimensional electrophoresis for the fractionation of royal jelly proteins was performed, and the zinc level was quantified by the flame atomic absorption spectrometry (FAAS) technique. Proteins were identified by electrospray ionization mass spectrometry (ESI MS MS). Analysis of variance followed by the Tukey test (P < 0.05) was used. Supplementation with the mineral zinc positively affected the quantification of proteins for treatments 50 and 75 ppm. However, all treatments independent of zinc concentrations showed fewer protein spots when compared to the control. All zinc-containing proteins were classified as major royal jelly proteins (MRJPs). The exposure of nursing bees to the mineral zinc in its inorganic form reduced the expression of six different MRJPs involved in larval and glands development of nursing bees (MRJP1, MRJP2, MRJP3, MRJP5, and MRJP7), however promoted an increase in the expression of royal jelly proteins involved in defense systems (MRJP8 and MRJP9). The results demonstrate that vital proteins and metabolic processes are impaired in nursing bees exposed to the mineral zinc in its inorganic form in all doses used affecting nutrition and maintenance of colonies.en
dc.description.affiliationFaculty of Veterinary Medicine and Animal Science UNESP- São Paulo State University
dc.description.affiliationInstitute of Biosciences of Botucatu UNESP - São Paulo State University
dc.description.affiliationUnespFaculty of Veterinary Medicine and Animal Science UNESP- São Paulo State University
dc.description.affiliationUnespInstitute of Biosciences of Botucatu UNESP - São Paulo State University
dc.identifierhttp://dx.doi.org/10.1007/s12011-020-02564-3
dc.identifier.citationBiological Trace Element Research.
dc.identifier.doi10.1007/s12011-020-02564-3
dc.identifier.issn1559-0720
dc.identifier.issn0163-4984
dc.identifier.scopus2-s2.0-85098973691
dc.identifier.urihttp://hdl.handle.net/11449/205692
dc.language.isoeng
dc.relation.ispartofBiological Trace Element Research
dc.sourceScopus
dc.subjectBees
dc.subjectMineral
dc.subjectProteomic
dc.subjectSupplementation
dc.titleSupplementation with an Inorganic Zinc Source in the Metalloproteomic Profile of Royal Jelly in Apis mellifera L.en
dc.typeArtigo

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