Temperature Gradient Gel Electrophoresis as a Valuable Accessory Tool for Assessment of Dysbiosis in Crohn's Disease

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dc.contributor.authorCarvalho, Vanessa Rafaela de [UNESP]
dc.contributor.authorKeller, Rogeria [UNESP]
dc.contributor.authorSilva Santos, Ana Carolina da [UNESP]
dc.contributor.authorRodrigues, Josias [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Western Sao Paulo
dc.date.accessioned2018-11-26T17:40:09Z
dc.date.available2018-11-26T17:40:09Z
dc.date.issued2016-07-01
dc.description.abstractEscherichia coli and other Proteobacteria are augmented and several other bacteria are diminished in Crohn's (CD) disease patients' intestine. This imbalance in bacterial species composition-termed dysbiosis-seems to be determinant of CD manifestation. Since a great part of intestinal bacteria are not cultivable, detection of CD dysbiosis is accomplished by molecular tools, involving sequences analysis of the 16SrRNA gene (16SrDNA) present in the patient's clinical samples, which can be done by sequencing or electrophoresis in denaturing gels of 16SrDNA amplicons. By analyzing, by temperature gradient gel electrophoresis (TGGE) and next generation sequencing of 16SV6-V8rDNA amplicons present in gram negative cultures from four distinct clinical samples of a control subject and a CD patient, this study demonstrates that both techniques were able to detect E. coli over-growth and reduction in species richness in CD and that TGGE can discriminate sequences collectively labeled as unclassified in 16SrDNA databases. Although TGGE per se does not identify the sequences, the discriminatory power that it confers represents valuable accessory information to next generation DNA sequencing (NGS), and as such must be used as a NGS complementary tool.en
dc.description.affiliationState Univ Sao Paulo, UNESP, Lab Med Bacteriol, Dept Microbiol & Immunol,Inst Biosci, Botucatu, SP, Brazil
dc.description.affiliationUniv Western Sao Paulo, Presidente Prudente, Brazil
dc.description.affiliationUnespState Univ Sao Paulo, UNESP, Lab Med Bacteriol, Dept Microbiol & Immunol,Inst Biosci, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/04475-3
dc.format.extent549-554
dc.identifierhttp://dx.doi.org/10.4236/aim.2016.68055
dc.identifier.citationAdvances In Microbiology. Irvine: Hans Publishers, v. 6, n. 8, p. 549-554, 2016.
dc.identifier.doi10.4236/aim.2016.68055
dc.identifier.fileWOS000407163300001.pdf
dc.identifier.issn2327-0810
dc.identifier.urihttp://hdl.handle.net/11449/163110
dc.identifier.wosWOS:000407163300001
dc.language.isoeng
dc.publisherHans Publishers
dc.relation.ispartofAdvances In Microbiology
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectDysbiosis
dc.subjectProteobacteria
dc.subjectEscherichia coli
dc.subjectCrohn's Disease
dc.titleTemperature Gradient Gel Electrophoresis as a Valuable Accessory Tool for Assessment of Dysbiosis in Crohn's Diseaseen
dc.typeArtigo
dcterms.rightsHolderHans Publishers
unesp.author.orcid0000-0001-8480-672X[4]

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Artigos - Microbiologia e Imunologia - IBB