Anti-inflammatory action of ethanolic extract and clerodane diterpenes from Casearia sylvestris

dc.contributor.authorPierri, Elaise G. [UNESP]
dc.contributor.authorCastro, Rogerio C. [UNESP]
dc.contributor.authorVizioli, Ednir O. [UNESP]
dc.contributor.authorFerreira, Carla M. R. [UNESP]
dc.contributor.authorCavalheiro, Alberto J. [UNESP]
dc.contributor.authorTininis, Aristeu G.
dc.contributor.authorChin, Chung M. [UNESP]
dc.contributor.authorSantos, Andre G. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Fed Sao Paulo
dc.date.accessioned2018-11-26T17:40:05Z
dc.date.available2018-11-26T17:40:05Z
dc.date.issued2017-07-01
dc.description.abstractThe present study aimed to investigate the anti-inflammatory activity of ethanolic extract from Casearia sylvestris Sw., Salicaceae, leaves and to identify the compounds responsible for this activity. The ethanolic extract from C. sylvestris leaves was fractionated by solid phase extraction and the chemical composition of extract and fractions were assessed by chromatographic techniques. Casearin-like clerodane diterpenes were quantified in ethanolic extract (27.4%, w/w) and in fraction 2 of solid phase extraction (50.6%, w/w). Carrageenan-induced paw edema and carrageenan-induced pleurisy assays (rats) were used to evaluate anti-inflammatory activity of ethanolic extract, its fractions and clerodane diterpenes from C. sylvestris caseargrewiin F and casearin B. The ethanolic extract was tested in the rat paw edema model and the doses tested (10 and 100 mg/kg) had no effect. In the pleurisy model, the extract doses of 300 and 500 mg/kg showed inhibitory effect. The fraction 2 of solid phase extraction (10 mg/kg), caseargrewiin F and casearin B (0.5 mg/kg) showed a significant reduction (p < 0.05) of the carrageenan-induced paw edema in rats compared to indomethacin. Gastric ulcers were not observed in animals treated with samples from C. sylvestris. In conclusion, the ethanolic extract from C. sylvestris, its enriched fraction of clerodane diterpenes, casearin B and caseargrewiin F exhibited anti-inflammatory activity on in vivo models in rats. Casearin-like clerodane diterpenes may be considered active chemical markers for C. sylvestris leaves. On the other hand, these diterpenes are promising compounds in the development of new drugs with anti-inflammatory action without gastric side effects. (c) 2017 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Principios Ativos Nat & Toxicol, Lab Farmacognosia, Araraquara, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Farm & Med, Lab Pesquisa Desenvolvimento Farm, Araraquara, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Dept Quim Organ, Nucleo Bioensaios Biossintese & Ecofisiol Prod Na, Araraquara, SP, Brazil
dc.description.affiliationInst Fed Sao Paulo, Campus Avancado Matao, Matao, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Principios Ativos Nat & Toxicol, Lab Farmacognosia, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Farm & Med, Lab Pesquisa Desenvolvimento Farm, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Dept Quim Organ, Nucleo Bioensaios Biossintese & Ecofisiol Prod Na, Araraquara, SP, Brazil
dc.format.extent495-501
dc.identifierhttp://dx.doi.org/10.1016/j.bjp.2016.12.008
dc.identifier.citationRevista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosy. Curitiba-pr: Soc Brasileira Farmacognosia, v. 27, n. 4, p. 495-501, 2017.
dc.identifier.doi10.1016/j.bjp.2016.12.008
dc.identifier.fileS0102-695X2017000400495.pdf
dc.identifier.issn0102-695X
dc.identifier.lattes9734333607975413
dc.identifier.orcid0000-0003-4141-0455
dc.identifier.scieloS0102-695X2017000400495
dc.identifier.urihttp://hdl.handle.net/11449/163098
dc.identifier.wosWOS:000406961700015
dc.language.isoeng
dc.publisherSoc Brasileira Farmacognosia
dc.relation.ispartofRevista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosy
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectClerodane diterpene
dc.subjectCasearin B
dc.subjectCaseargrewiin F
dc.subjectAnti-inflammatory activity
dc.subjectGastric side effects
dc.titleAnti-inflammatory action of ethanolic extract and clerodane diterpenes from Casearia sylvestrisen
dc.typeArtigo
dcterms.rightsHolderSoc Brasileira Farmacognosia
unesp.author.lattes2518006820764120[5]
unesp.author.lattes4000625974516852[8]
unesp.author.lattes9734333607975413[7]
unesp.author.orcid0000-0001-8214-9957[5]
unesp.author.orcid0000-0002-4920-2506[8]
unesp.author.orcid0000-0003-4141-0455[7]

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