Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals

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dc.contributor.authorLopes, Mariana Mescouto
dc.contributor.authorBrito, Thaís Ribeiro [UNESP]
dc.contributor.authorLage, Josiane Fonseca
dc.contributor.authorCosta, Thaís Correia
dc.contributor.authorFontes, Marta Maria Dos Santos
dc.contributor.authorSerão, Nick Vergara Lopes
dc.contributor.authorMendes, Tiago Antônio de Oliveira
dc.contributor.authorReis, Ricardo Andrade [UNESP]
dc.contributor.authorVeroneze, Renata
dc.contributor.authorE Silva, Fabyano Fonseca
dc.contributor.authorDuarte, Marcio de Souza
dc.contributor.institutionUniversidade Federal de Viçosa (UFV)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionResearch and Development
dc.contributor.institutionIowa State University
dc.date.accessioned2022-05-01T05:29:35Z
dc.date.available2022-05-01T05:29:35Z
dc.date.issued2021-07-01
dc.description.abstractVitamin B and trace minerals are crucial molecular signals involved in many biological pathways; however, their bioavailability is compromised in high-producing ruminant animals. So far, studies have mainly focused on the effects of these micronutrients on animal performance, but their use in a rumen-protected form and their impact on liver metabolism in finishing beef cattle is poorly known. We used a shotgun proteomic approach combined with biological network analyses to assess the effects of a rumen-protected B-vitamin blend, as well as those of hydroxy trace minerals, on the hepatic proteome. A total of 20 non-castrated Nellore males with 353 ± 43 kg of initial body weight were randomly assigned to one of the following treatments: CTRL—inorganic trace minerals without supplementation of a protected vitamin B blend, or SUP—supplementation of hydroxy trace minerals and a protected vitamin B blend. All animals were fed the same amount of the experimental diet for 106 days, and liver biopsies were performed at the end of the experimental period. Supplemented animals showed 37 up-regulated proteins (p < 0.10), and the enrichment analysis revealed that these proteins were involved in protein folding (p = 0.04), mitochondrial respiratory chain complex I (p = 0.01) and IV (p = 0.01), chaperonin-containing T-complex 2 (p = 0.01), glutathione metabolism (p < 0.01), and other aspects linked to oxidative-stress responses. These results indicate that rumen-protected vitamin B and hydroxy trace mineral supplementation during the finishing phase alters the abundance of proteins associated with the electron transport chain and other oxidation–reduction pathways, boosting the production of reactive oxygen species, which appear to modulate proteins linked to oxidative-damage responses to maintain cellular homeostasis.en
dc.description.affiliationDepartment of Animal Science Universidade Federal de Viçosa
dc.description.affiliationMuscle Biology and Nutrigenomics Laboratory Universidade Federal de Viçosa
dc.description.affiliationDepartment of Animal Science São Paulo State University
dc.description.affiliationTrouw Nutrition Research and Development
dc.description.affiliationDepartment of Animal Science Iowa State University
dc.description.affiliationDepartment of Biochemistry and Molecular Biology Universidade Federal de Viçosa
dc.description.affiliationUnespDepartment of Animal Science São Paulo State University
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdCNPq: 31883/2018-4
dc.identifierhttp://dx.doi.org/10.3390/ani11071934
dc.identifier.citationAnimals, v. 11, n. 7, 2021.
dc.identifier.doi10.3390/ani11071934
dc.identifier.issn2076-2615
dc.identifier.scopus2-s2.0-85108706060
dc.identifier.urihttp://hdl.handle.net/11449/233197
dc.language.isoeng
dc.relation.ispartofAnimals
dc.sourceScopus
dc.subjectBeef cattle
dc.subjectHydroxyl trace minerals
dc.subjectLiver metabolism
dc.subjectProteomics
dc.subjectVitamin B
dc.titleProteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace mineralsen
dc.typeArtigo

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