MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis

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dc.contributor.authorDe Freitas, Jéssica Henrique [UNESP]
dc.contributor.authorBragato, Jaqueline Poleto [UNESP]
dc.contributor.authorRebech, Gabriela Torres [UNESP]
dc.contributor.authorCosta, Sidnei Ferro [UNESP]
dc.contributor.authorDos Santos, Marilene Oliveira [UNESP]
dc.contributor.authorSoares, Matheus Fujimura [UNESP]
dc.contributor.authorEugênio, Flávia de Rezende [UNESP]
dc.contributor.authorDos Santos, Paulo Sérgio Patto [UNESP]
dc.contributor.authorDe Lima, Valéria Marçal Felix [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T16:12:19Z
dc.date.available2023-07-29T16:12:19Z
dc.date.issued2023-01-01
dc.description.abstractCanine Visceral leishmaniasis (CanL) poses a severe public health threat in several countries. Disease progression depends on the degree of immune response suppression. MicroRNAs (miRs) modulate mRNA translation into proteins and regulate various cellular functions and pathways associated with immune responses. MiR-21 and miR-148a can alter the parasite load and M1 macrophages are the principal cells in dogs’ leishmanicidal activity. A previous study found increased miR-21 and miR-148a in splenic leukocytes (SL) of dogs with CanL using microarray analysis and in silico analysis identified PTEN pathway targets. PTEN is involved in the immune regulation of macrophages. We measured PTEN and the production of reactive oxygen species (ROS) and nitric oxide (NO) before and after transfection SLs of dogs with CanL with mimic and inhibition of miR-21 and miR-148a. PTEN levels increased, NO and ROS decreased in SLs from dogs with CanL. Inhibition of miRNA-21 resulted in PTEN increase; in contrast, PTEN decreased after miR-148a inhibition. Nitrite (NO2) levels increased after transfection with miR-21 inhibitor but were decreased with miR-148a inhibitor. The increase in miR-21 promoted a reduction in ROS and NO levels, but miR-148a inhibition increased NO and reduced ROS. These findings suggest that miR-21 and miR-148a can participate in immune response in CanL, affecting PTEN, NO, and ROS levels.en
dc.description.affiliationDepartment of Animal Clinic School of Veterinary Medicine Surgery and Reproduction São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Animal Clinic School of Veterinary Medicine Surgery and Reproduction São Paulo State University (UNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2018/17261-5 2021/07283-4
dc.identifierhttp://dx.doi.org/10.3389/fgene.2023.1106496
dc.identifier.citationFrontiers in Genetics, v. 14.
dc.identifier.doi10.3389/fgene.2023.1106496
dc.identifier.issn1664-8021
dc.identifier.scopus2-s2.0-85153709256
dc.identifier.urihttp://hdl.handle.net/11449/249899
dc.language.isoeng
dc.relation.ispartofFrontiers in Genetics
dc.sourceScopus
dc.subjectLeishmania infantum
dc.subjectmicroRNA
dc.subjectnitrite
dc.subjectNO
dc.subjectpten
dc.subjectROS
dc.subjectsplenic leukocytes
dc.titleMicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasisen
dc.typeArtigo

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Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA