Ability of donkey sperm to tolerate cooling: Effect of extender base and removal of seminal plasma on sperm parameters and fertility rates in mares

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dc.contributor.authorGobato, Mariana L. M. [UNESP]
dc.contributor.authorSegabinazzi, Lorenzo G. T. M. [UNESP]
dc.contributor.authorScheeren, Verônica F. C. [UNESP]
dc.contributor.authorBandeira, Rafael S. [UNESP]
dc.contributor.authorFreitas-Dell'Aqua, Camila P. [UNESP]
dc.contributor.authorDell'Aqua, José A. [UNESP]
dc.contributor.authorPapa, Frederico O. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionRoss University School of Veterinary Medicine
dc.date.accessioned2023-07-29T14:51:48Z
dc.date.available2023-07-29T14:51:48Z
dc.date.issued2022-09-26
dc.description.abstractArtificial insemination using cooled-transported semen has marked importance in equine breeding programs around the world, and the high value of mules has generated avid interest in donkey semen biotechnology. However, donkey semen cools poorly in commercially available equine extenders. Therefore, this study aimed to develop approaches to improve the ability of donkey semen to tolerate cooling. Ejaculates of seven donkeys (n = 21) were cooled at 5°C for 48 h in three different extenders (milk-based, SM; sodium caseinate-based, SC; or egg yolk-based, EY) in the presence or absence of seminal plasma (centrifugation, C). Sperm motility, plasma membrane integrity (PMI), plasma membrane stability (PMS), mitochondrial membrane potential (HMMP), intracellular hydrogen peroxide (H2O2), and intracellular superoxide ((Formula presented.)) were assessed before, 24 h, and 48 h post-cooling. In addition, 15 mares (163 estrous cycles) were randomly inseminated with semen from two jacks (Jack 1, n = 90; Jack 2, n = 73) previously cooled for 24 h under one of the treatments (SM, SC, EY, SM-C, SC-C, or EY-C). Groups EY, SC-C, and EY-C (P < 0.05) demonstrated superior sperm analytical parameters to SM at 24 and 48 h. Centrifugation positively affected sperm analytical parameters in cooled donkey semen extended in SM and SC (P < 0.05). Mares bred with semen extended in SC (67%, 18/27), SC-C (89%, 24/27), EY (89%, 25/28), or EY-C (74%, 20/27) had significantly greater conception rates than mares bred with SM (33%, 9/27; P < 0.05). Mares bred with SM-C had intermediate conception rates (59%, 16/27). In conclusion, SC and EY improved the cooling ability and fertility of donkey semen in horse mares, and centrifugation positively affected donkey semen extended in SM.en
dc.description.affiliationSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP), São Paulo
dc.description.affiliationRoss University School of Veterinary Medicine
dc.description.affiliationUnespSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP), São Paulo
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.identifierhttp://dx.doi.org/10.3389/fvets.2022.1011899
dc.identifier.citationFrontiers in Veterinary Science, v. 9.
dc.identifier.doi10.3389/fvets.2022.1011899
dc.identifier.issn2297-1769
dc.identifier.scopus2-s2.0-85139459619
dc.identifier.urihttp://hdl.handle.net/11449/249239
dc.language.isoeng
dc.relation.ispartofFrontiers in Veterinary Science
dc.sourceScopus
dc.subjectartificial insemination
dc.subjectequine
dc.subjectjack
dc.subjectmule
dc.subjectstallion
dc.titleAbility of donkey sperm to tolerate cooling: Effect of extender base and removal of seminal plasma on sperm parameters and fertility rates in maresen
dc.typeArtigo

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