Megagametophyte development and female sterility in Maytenus obtusifolia Mart. (Celastraceae)

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dc.contributor.authorNader Haddad, Isabella Verissimo
dc.contributor.authorSa-Haiad, Barbara de
dc.contributor.authorRibeiro de Santiago-Fernandes, Lygia Dolores
dc.contributor.authorMachado, Silvia Rodrigues [UNESP]
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-10T19:42:32Z
dc.date.available2020-12-10T19:42:32Z
dc.date.issued2019-11-01
dc.description.abstractReproduction in flowering plants is closely related to the megagametophyte, since the megagametophyte is involved in pollen tube reception and contains the two female gametes-egg cell and central cell. Previous conventional light microscopy methods have shown that female sterility in perfect flowers of Maytenus obtusifolia is associated with the occurrence of sterile ovules whose megagametophytes have hypertrophied synergids. Here, using transmission electron microscopy and cytochemical methods, we compare the megagametophytes in fertile and sterile ovules from perfect and pistillate flowers, and investigate the cellular events that result in the degradation of the megagametophyte cells from sterile ovules. In fertile ovules of perfect and pistillate flowers, mature megagametophytes have two synergids, egg cell and central cell. In fertile ovules, the synergids present an extensive rough endoplasmic reticulum (RER) profile, large populations of mitochondria, when compared to egg cells, vesicles, Golgi bodies, plastids and a nucleus with heterochromatin. Besides that, the egg cell has a small population of organelles and the central cell exhibits cytoplasm with free ribosomes, RER, vesicles originating from the RER, Golgi bodies and oil inclusions. In mature megagametophytes from sterile ovules of perfect and pistillate flowers, massive autophagy occurs by tonoplast rupture promoting hydrolase release, leading to protoplast and cell wall degradation-typical evidence of programmed cell death (PCD). Therefore, female sterility in the majority of M. obtusifolia sterile ovules is the result of PCD by massive autophagy in the megagametophyte cells. In a few other sterile ovules, sterility is due to the delayed or the absence of megagametophyte development.en
dc.description.affiliationUniv Fed Rio de Janeiro, Museu Nacl, Dept Bot, BR-22940040 Rio De Janeiro, RJ, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Bot, BR-18618000 Sao Paulo, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Bot, BR-18618000 Sao Paulo, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipIdCNPq: 304396/20150
dc.description.sponsorshipIdFAPERJ: E-26/111.207/2014
dc.description.sponsorshipIdCNPq: 447624/2014-8
dc.format.extent1667-1680
dc.identifierhttp://dx.doi.org/10.1007/s00709-019-01413-y
dc.identifier.citationProtoplasma. Wien: Springer Wien, v. 256, n. 6, p. 1667-1680, 2019.
dc.identifier.doi10.1007/s00709-019-01413-y
dc.identifier.issn0033-183X
dc.identifier.urihttp://hdl.handle.net/11449/196370
dc.identifier.wosWOS:000500030400017
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofProtoplasma
dc.sourceWeb of Science
dc.subjectAutophagy
dc.subjectCytochemistry
dc.subjectProgrammed cell death (PCD)
dc.subjectUltrastructure
dc.subjectVacuolar cell death
dc.titleMegagametophyte development and female sterility in Maytenus obtusifolia Mart. (Celastraceae)en
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer

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