A biocomplex to repair experimental critical size defects associated with photobiomodulation therapy

Página do item simplificado
dc.contributor.authorBuchaim, Daniela Vieira [UNESP]
dc.contributor.authorAndreo, Jesus Carlos
dc.contributor.authorPomini, Karina Torres
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.authorFerreira, Rui Seabra [UNESP]
dc.contributor.authorDuarte, Marco Antonio Hungaro
dc.contributor.authorAlcalde, Murilo Priori
dc.contributor.authorReis, Carlos Henrique Bertoni
dc.contributor.authorde Bortoli Teixeira, Daniel
dc.contributor.authorde Souza Bueno, Cleuber Rodrigo
dc.contributor.authorDetregiachi, Cláudia Rucco Penteado
dc.contributor.authorAraujo, Adriano Cressoni
dc.contributor.authorBuchaim, Rogério Leone [UNESP]
dc.contributor.institutionUniversity of Marilia (UNIMAR)
dc.contributor.institutionUniversity Center of Adamantina (UniFAI)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUnisagrado University Center
dc.date.accessioned2022-04-29T08:40:40Z
dc.date.available2022-04-29T08:40:40Z
dc.date.issued2022-01-01
dc.description.abstractBackground: The association of scaffolds to repair extensive bone defects can contribute to their evolution and morphophysiological recomposition. The incorporation of particulate biomaterials into three-dimensional fibrin bioproducts together with photobiomodulation therapy (PBM) has potential and can improve regenerative medicine procedures. The objective of this experiment was to evaluate the effects of PBM therapy on critical size defects filled with xenogenic bone substitute associated with fibrin biopolymer. Methods: A critical defect of 8 mm was performed in 36 Wistar male adult rats that were divided into four groups. Groups BC and BC-PBM were defined as controls with defects filled by a clot (without or with PBM, respectively) and groups XS and XS-PBM that comprised those filled with biocomplex Bio-OssTM in association with fibrin biopolymer. PBM was applied immediately after the surgery and three times a week every other day, with the parameters: wavelength of 830 nm, energy density 6.2 J/cm2, output power 30 mW, beam area of 0.116 cm2, irradiance 0.258,62 W/cm2, energy/point 0.72 J, total energy 2.88 J. Fourteen and 42 days after the surgery, animals were euthanatized and subjected to microtomography, qualitative and quantitative histological analysis. Results: The BC-PBM and XS-PBM groups had a similar evolution in the tissue repair process, with a higher density of the volume of new formed bone in relation to the groups without PBM (p = 0.04086; p = 0.07093, respectively). Intense vascular proliferation and bone deposition around the biomaterial particles were observed in the animals of the groups in which biocomplex was applied (XS and XS-PBM). Conclusion: PBM therapy allowed an improvement in the formation of new bone, with a more organized deposition of collagen fibers in the defect area. Biocomplex favored the insertion and permanence of the particulate material in bone defects, creating a favorable microenvironment for accelerate repair process.en
dc.description.affiliationGraduate Program in Structural and Functional Interactions in Rehabilitation University of Marilia (UNIMAR), SP
dc.description.affiliationMedical School University Center of Adamantina (UniFAI), SP
dc.description.affiliationCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Biological Sciences (Anatomy) Bauru School of Dentistry University of São Paulo (USP), SP
dc.description.affiliationGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP), SP
dc.description.affiliationGraduate Program in Clinical Research Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São Paulo (USP), SP
dc.description.affiliationGraduate Program in Applied Dental Sciences Bauru School of Dentistry University of São Paulo (USP), SP
dc.description.affiliationDepartment of Health Science Unisagrado University Center, SP
dc.description.affiliationUnespCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP), SP
dc.description.affiliationUnespGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP), SP
dc.description.affiliationUnespGraduate Program in Clinical Research Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP), SP
dc.identifierhttp://dx.doi.org/10.1590/1678-9199-JVATITD-2021-0056
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases, v. 28.
dc.identifier.doi10.1590/1678-9199-JVATITD-2021-0056
dc.identifier.issn1678-9199
dc.identifier.issn1678-9180
dc.identifier.scopus2-s2.0-85125934972
dc.identifier.urihttp://hdl.handle.net/11449/230532
dc.language.isoeng
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.sourceScopus
dc.subjectBiomaterials
dc.subjectBone regeneration
dc.subjectBone substitutes
dc.subjectFibrin sealant
dc.subjectLow-level laser therapy
dc.subjectPhotobiomodulation therapy
dc.titleA biocomplex to repair experimental critical size defects associated with photobiomodulation therapyen
dc.typeArtigo

Arquivos

Coleções

Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos
Artigos - Doenças Tropicais e Diagnósticos por Imagem - FMB