Molecular Individual-Based Approach on Triatoma brasiliensis: Inferences on Triatomine Foci, Trypanosoma cruzi Natural Infection Prevalence, Parasite Diversity and Feeding Sources

dc.contributor.authorAlmeida, Carlos Eduardo [UNESP]
dc.contributor.authorFaucher, Leslie
dc.contributor.authorLavina, Morgane
dc.contributor.authorCosta, Jane
dc.contributor.authorHarry, Myriam
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversité Paris-Saclay
dc.contributor.institutionUniversidade Federal da Paraíba (UFPB)
dc.contributor.institutionInstituto Oswaldo Cruz – Fiocruz
dc.contributor.institutionUniversité Paris-Sud
dc.description.abstractWe used an individual-based molecular multisource approach to assess the epidemiological importance of Triatoma brasiliensis collected in distinct sites and ecotopes in Rio Grande do Norte State, Brazil. In the semi-arid zones of Brazil, this blood sucking bug is the most important vector of Trypanosoma cruzi—the parasite that causes Chagas disease. First, cytochrome b (cytb) and microsatellite markers were used for inferences on the genetic structure of five populations (108 bugs). Second, we determined the natural T. cruzi infection prevalence and parasite diversity in 126 bugs by amplifying a mini-exon gene from triatomine gut contents. Third, we identified the natural feeding sources of 60 T. brasiliensis by using the blood meal content via vertebrate cytb analysis. Demographic inferences based on cytb variation indicated expansion events in some sylvatic and domiciliary populations. Microsatellite results indicated gene flow between sylvatic and anthropic (domiciliary and peridomiciliary) populations, which threatens vector control efforts because sylvatic population are uncontrollable. A high natural T. cruzi infection prevalence (52–71%) and two parasite lineages were found for the sylvatic foci, in which 68% of bugs had fed on Kerodon rupestris (Rodentia: Caviidae), highlighting it as a potential reservoir. For peridomiciliary bugs, Galea spixii (Rodentia: Caviidae) was the main mammal feeding source, which may reinforce previous concerns about the potential of this animal to link the sylvatic and domiciliary T. cruzi cycles.en
dc.description.affiliationDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas (UNESP)
dc.description.affiliationUMR EGCE (Evolution Genome Comportment Ecologie) CNRS-IRD-Univ. Paris-Sud IDEEV Université Paris-Saclay
dc.description.affiliationPrograma de Pós-Graduação em Ecologia e Monitoramento Ambiental – PPGEMA Universidade Federal da Paraíba PB
dc.description.affiliationLaboratório de Biodiversidade Entomológica Instituto Oswaldo Cruz – Fiocruz
dc.description.affiliationUniversité Paris-Sud
dc.description.affiliationUnespDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCentre National de la Recherche Scientifique
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação Oswaldo Cruz
dc.description.sponsorshipIdFAPESP: 2010/17027-0
dc.description.sponsorshipIdFAPESP: 2011/22378-0
dc.identifier.citationPLoS Neglected Tropical Diseases, v. 10, n. 2, 2016.
dc.relation.ispartofPLoS Neglected Tropical Diseases
dc.rights.accessRightsAcesso aberto
dc.titleMolecular Individual-Based Approach on Triatoma brasiliensis: Inferences on Triatomine Foci, Trypanosoma cruzi Natural Infection Prevalence, Parasite Diversity and Feeding Sourcesen
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