Inflammatory profile of apical periodontitis associated with liver fibrosis in rats: histological and immunohistochemical analysis

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dc.contributor.authorCantiga-Silva, C. [UNESP]
dc.contributor.authorEstrela, C.
dc.contributor.authorSegura-Egea, J. J.
dc.contributor.authorAzevedo, J. P. [UNESP]
dc.contributor.authorde Oliveira, P. H.C. [UNESP]
dc.contributor.authorCardoso, C. B.M. [UNESP]
dc.contributor.authorPinheiro, T. N.
dc.contributor.authorErvolino, E. [UNESP]
dc.contributor.authorSivieri-Araújo, G. [UNESP]
dc.contributor.authorCintra, L. T.A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Goiás (UFG)
dc.contributor.institutionUniversity of Sevilla
dc.contributor.institutionDental School of Amazonas State University
dc.date.accessioned2021-06-25T10:57:44Z
dc.date.available2021-06-25T10:57:44Z
dc.date.issued2021-01-01
dc.description.abstractAim: To investigate the effects of liver fibrosis (LF) on the pro-inflammatory mediators and periapical bone resorption of apical periodontitis (AP) in rats. Methodology: Forty male Wistar rats were distributed into four groups: C – control, AP – rats with AP, LF – rats with LF, AP + LF – rats with AP and LF. LF was induced by carbon tetrachloride administration for 8 weeks and surgical bile duct ligation for 4 weeks; AP was induced in the teeth of rats by dental pulp exposure to the oral environment for 30 days. Jaws and livers were removed after euthanasia. Haematoxylin and Eosin (H&E) and Picrosirius Red (PSR) staining were used to confirm fibrosis in the livers. The jaws were analysed using H&E staining, immunohistochemical assays of interleukin (IL)-1β, IL-6 and tumour necrosis factor-alpha (TNF-α). Student’s t-test and Mann–Whitney’s U-test were used for statistical analysis (P < 0.05). Results: Inflammatory infiltrate was moderate in the AP group and severe in the AP + LF group (P < 0.05). Periapical bone resorption was significantly larger in the AP + LF group compared with the AP group (P < 0.05). IL-1β, IL-6 and TNF-α levels were significantly higher in AP + LF group when compared to the AP group (P < 0.05). Conclusion: More intense inflammatory infiltrate, greater amounts of pro-inflammatory cytokines and increased periapical bone resorption were observed in the presence of liver fibrosis in rats with exposed pulps.en
dc.description.affiliationEndodontic Section Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Stomatologic Sciences School of Dentistry UFG – Federal University of Goiás
dc.description.affiliationEndodontic Section Department of Stomatology School of Dentistry University of Sevilla
dc.description.affiliationDepartment of Oral Pathology and Oral Medicine Dental School of Amazonas State University
dc.description.affiliationDepartment of Basic Science School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDental Assistance Center for Disabled Persons (CAOE) School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespEndodontic Section Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Basic Science School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespDental Assistance Center for Disabled Persons (CAOE) School of Dentistry São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.1111/iej.13519
dc.identifier.citationInternational Endodontic Journal.
dc.identifier.doi10.1111/iej.13519
dc.identifier.issn1365-2591
dc.identifier.issn0143-2885
dc.identifier.scopus2-s2.0-85103981159
dc.identifier.urihttp://hdl.handle.net/11449/207586
dc.language.isoeng
dc.relation.ispartofInternational Endodontic Journal
dc.sourceScopus
dc.subjectapical periodontitis
dc.subjectcytokines
dc.subjectinflammation
dc.subjectliver fibrosis
dc.subjectoral infection
dc.titleInflammatory profile of apical periodontitis associated with liver fibrosis in rats: histological and immunohistochemical analysisen
dc.typeArtigo
unesp.author.orcid0000-0003-4313-7149[1]
unesp.author.orcid0000-0002-1488-0366[2]
unesp.author.orcid0000-0002-0427-9059[3]
unesp.author.orcid0000-0001-9161-0549[4]
unesp.author.orcid0000-0002-1593-7926[5]
unesp.author.orcid0000-0002-4943-7022[6]
unesp.author.orcid0000-0003-2987-4759[7]
unesp.author.orcid0000-0003-4859-0583[8]
unesp.author.orcid0000-0002-8402-7408[9]
unesp.author.orcid0000-0003-2348-7846[10]

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