Crystallization and preliminary X-ray crystallographic studies of a Lys49-phospholipase A(2) homologue from Bothrops pirajai venom complexed with rosmarinic acid

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dc.contributor.authordos Santos, Juliana I. [UNESP]
dc.contributor.authorSantos-Filho, Norival A.
dc.contributor.authorSoares, Andreimar M.
dc.contributor.authorFontes, Marcos R. M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:49:39Z
dc.date.available2014-05-20T13:49:39Z
dc.date.issued2010-06-01
dc.description.abstractPrTX-I, a noncatalytic and myotoxic Lys49-phospholipase A(2) from Bothrops pirajai venom, was crystallized in the presence of the inhibitor rosmarinic acid (RA). This is the active compound in the methanolic extract of Cordia verbenacea, a plant that is largely used in Brazilian folk medicine. The crystals diffracted X-rays to 1.8 angstrom resolution and the structure was solved by molecular-replacement techniques, showing electron density that corresponds to RA molecules at the entrance to the hydrophobic channel. The crystals belong to space group P2(1)2(1)2(1), indicating conformational changes in the structure after ligand binding: the crystals of all apo Lys49-phospholipase A(2) structures belong to space group P3(1)2(1), while the crystals of complexed structures belong to space groups P2(1) or P2(1)2(1)2(1).en
dc.description.affiliationUniv Estadual Paulista, Dept Fis Biofis, Inst Biociencias, UNESP, Botucatu, SP, Brazil
dc.description.affiliationUSP, Dept Anal Clin Toxicol & Bromatol, FCFRP, Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Fis Biofis, Inst Biociencias, UNESP, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipLaboratório Nacional de Luz Síncrotron (LNLS)
dc.format.extent699-701
dc.identifierhttp://dx.doi.org/10.1107/S1744309110013709
dc.identifier.citationActa Crystallographica Section F-structural Biology and Crystallization Communications. Malden: Wiley-blackwell, v. 66, p. 699-701, 2010.
dc.identifier.doi10.1107/S1744309110013709
dc.identifier.fileWOS000278165900017.pdf
dc.identifier.issn1744-3091
dc.identifier.urihttp://hdl.handle.net/11449/17703
dc.identifier.wosWOS:000278165900017
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofActa Crystallographica Section F: Structural Biology and Crystallization Communications
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleCrystallization and preliminary X-ray crystallographic studies of a Lys49-phospholipase A(2) homologue from Bothrops pirajai venom complexed with rosmarinic aciden
dc.typeArtigo
dcterms.rightsHolderWiley-blackwell
unesp.author.orcid0000-0002-0344-6900[2]
unesp.author.orcid0000-0002-4634-6221[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Física e Biofísica - IBB