A polymerase chain reaction for detection of Brucella canis in vaginal swabs of naturally infected bitches

dc.contributor.authorKeid, L. B.
dc.contributor.authorSoares, R. M.
dc.contributor.authorVasconcellos, S. A.
dc.contributor.authorChiebao, D. P.
dc.contributor.authorSalgado, V. R.
dc.contributor.authorMegid, Jane [UNESP]
dc.contributor.authorRichtzenhain, L. J.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionSecretaria Agr & Abastecimento Estado São Paulo
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:39:46Z
dc.date.available2014-05-20T13:39:46Z
dc.date.issued2007-12-01
dc.description.abstractA PCR assay for the detection of Brucella canis in canine vaginal swab samples was evaluated, comparing its performance with that of bacterial isolation, serological tests, and a blood PCR assay. One hundred and forty-four female dogs were clinically examined to detect reproductive problems and they were tested by the rapid slide agglutination test, with and without 2-mercaptoethanol (2ME-RSAT and RSAT, respectively). In addition, microbiological culture and PCR were performed on blood and vaginal swab samples. The results of the vaginal swab PCR were compared to those of the other tests using the Kappa coefficient and McNemar test. of the 144 females that were examined, 66 (45.8%) were RSAT positive, 23 (15.9%) were 2ME-RSAT positive, 49 (34.02%) were blood culture positive, 6 (4.1%) were vaginal swab culture positive, 54 (37.5%) were blood PCR positive, 52 (36.2%) were vaginal swab PCR positive, and 50.69% (73/144) were positive by the combined PCR. The PCR was able to detect as few as 3.8 fg of B. canis DNA experimentally diluted in 54 ng of canine DNA, extracted from vaginal swab samples of non-infected bitches. In addition, the PCR assay amplified B. canis genetic sequences from vaginal swab samples containing 1.0 x 10(0) cfu/mL. In conclusion, vaginal swab PCR was a good candidate as a confirmatory test for brucellosis diagnosis in bitches suspected to be infected, especially those negative on blood culture or blood PCR; these animals may be important reservoirs of infection and could complicate attempts to eradicate the disease in confined populations. (C) 2007 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Fac Med Vet & Zootecn, Dept Med Vet Prevent & Saúde Anim, BR-05508900 São Paulo, Brazil
dc.description.affiliationSecretaria Agr & Abastecimento Estado São Paulo, Agcy Paulista Tecnol Agronegocios, Sorocaba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Med Vet & Zootecn, Dept Higiene Vet & Saúde Publ, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Vet & Zootecn, Dept Higiene Vet & Saúde Publ, Botucatu, SP, Brazil
dc.format.extent1260-1270
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2007.08.021
dc.identifier.citationTheriogenology. New York: Elsevier B.V., v. 68, n. 9, p. 1260-1270, 2007.
dc.identifier.doi10.1016/j.theriogenology.2007.08.021
dc.identifier.issn0093-691X
dc.identifier.urihttp://hdl.handle.net/11449/13807
dc.identifier.wosWOS:000251030700008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcanine brucellosispt
dc.subjectvaginal swabpt
dc.subjectPCRpt
dc.subjectmicrobiological culturept
dc.subjectrapid slide agglutination testpt
dc.titleA polymerase chain reaction for detection of Brucella canis in vaginal swabs of naturally infected bitchesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-6540-7157[6]
unesp.author.orcid0000-0002-1419-3515[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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