On the interaction of small molecules with hemoproteins: Sperm whale myoglobin

dc.contributor.authorBaffa, O.
dc.contributor.authorTabak, M.
dc.contributor.authorNascimento, O. R.
dc.contributor.authorRuggiero, J. [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T00:10:55Z
dc.date.available2014-05-27T00:10:55Z
dc.date.issued1985-06-01
dc.description.abstractThe spin label TEMPO does not show a binding to myoglobin molecule in solution. This is probably due to the fact that this protein does not have a hydrophobic pocket large enough to accommodate the TEMPO molecule. In the crystal the spin label is bound and two kinds of spectra are observed: one isotropic and the other anisotropic. The anisotropic site is probably an intermolecular one. The correlation time for the label in the crystal is very sensitive to temperature showing a transition near 30 °C. This change can be explained as a result of the conformational change observed for myoglobin near this temperature: the motion of the spin label becomes more restricted below this temperature. Change in hydration is the probable cause of this structural change. The changes in the EPR spectra of the anisotropic label suggest that it is bound near the first layers of protein in the crystal. © 1985 Societá Italiana di Fisica.en
dc.description.affiliationFaculdade de Filosofia Ciencias e Letras-USP, Ribeirão Preto, 14.100, SP
dc.description.affiliationInstituto de Física e Química de São Carlos USP, São Carlos, C.P. 369, SP
dc.description.affiliationFaculdade de Filosofia, Ciéncias e Letras UNESP, São José do Rio Preto, 15.000, SP
dc.description.affiliationUnespFaculdade de Filosofia, Ciéncias e Letras UNESP, São José do Rio Preto, 15.000, SP
dc.format.extent516-526
dc.identifierhttp://dx.doi.org/10.1007/BF02452548
dc.identifier.citationIl Nuovo Cimento D, v. 5, n. 6, p. 516-526, 1985.
dc.identifier.doi10.1007/BF02452548
dc.identifier.issn0392-6737
dc.identifier.scopus2-s2.0-51249175097
dc.identifier.urihttp://hdl.handle.net/11449/63716
dc.language.isoeng
dc.relation.ispartofIl Nuovo Cimento D
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectMolecular biophysics
dc.titleOn the interaction of small molecules with hemoproteins: Sperm whale myoglobinen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights

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