Sublethal doses of imidacloprid and pyraclostrobin impair fat body of solitary bee Tetrapedia diversipes (Klug, 1810)

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dc.contributor.authorConceição de Assis, Josimere
dc.contributor.authorEduardo da Costa Domingues, Caio
dc.contributor.authorTadei, Rafaela [UNESP]
dc.contributor.authorInês da Silva, Cláudia
dc.contributor.authorSoares Lima, Hellen Maria
dc.contributor.authorDecio, Pâmela
dc.contributor.authorSilva-Zacarin, Elaine C.M.
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionFaculty of Agriculture and Life Sciences
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T19:52:25Z
dc.date.available2022-04-28T19:52:25Z
dc.date.issued2022-07-01
dc.description.abstractSolitary bees present greater species diversity than social bees. However, they are less studied than managed bees, mainly regarding the harmful effects of pesticides present in agroecosystems commonly visited by them. This study aimed to evaluate the effect of residual doses of imidacloprid and pyraclostrobin, alone and in combination, on the fat body (a multifunctional organ) of the neotropical solitary bee Tetrapedia diversipes by means of morphological and histochemical evaluation of oenocytes and trophocytes. Males and females of newly-emerged adults were submitted to bioassays of acute topical exposure. Experimental groups were essayed: control (CTR), solvent control (ACT), imidacloprid (IMI, 0.0028 ng/μL), pyraclostrobin (PYR, 2.7 ng/μL) and imidacloprid + pyraclostrobin (I + P). The data demonstrated that the residual doses applied in T. diversipes adults are sublethal at 96 h. Both oenocytes and trophocytes cells responded to topical exposure to the pesticides, showing morphological changes. In the IMI group, the bee oenocytes showed the greatest proportion of vacuolization and altered nuclei. The pyraclostrobin exposure increased the intensity of PAS-positive labeling (glycogen) in trophocytes. This increase was also observed in the I + P group. Changes in energy reserve (glycogen) of trophocytes indicate a possible mobilization impairment of this neutral polysaccharide to the hemolymph, which can compromise the fitness of exposed individuals. Also, changes in oenocytes can compromise the detoxification function performed by the fat body. This is the first study to show sublethal effects in neotropical solitary bees and highlight the importance of studies with native bees.en
dc.description.affiliationFederal University of São Carlos UFSCar Postgraduate Program in Biotechnology and Environmental Monitoring, São Paulo State
dc.description.affiliationUniversity of Maribor Faculty of Agriculture and Life Sciences
dc.description.affiliationSão Paulo State University UNESP Postgraduate Program in Biological Sciences, São Paulo State
dc.description.affiliationFederal University of São Carlos UFSCar Department of Biology Laboratory of Ecotoxicology and Environmental Integrity (LEIA), São Paulo State
dc.description.affiliationFederal University of São Carlos UFSCar Department of Environmental Sciences, São Paulo State
dc.description.affiliationUnespSão Paulo State University UNESP Postgraduate Program in Biological Sciences, São Paulo State
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2016/15743-7
dc.description.sponsorshipIdFAPESP: 2017/21097-3
dc.description.sponsorshipIdFAPESP: 2019/27863-5
dc.description.sponsorshipIdFAPESP: 2020/12639-0
dc.identifierhttp://dx.doi.org/10.1016/j.envpol.2022.119140
dc.identifier.citationEnvironmental Pollution, v. 304.
dc.identifier.doi10.1016/j.envpol.2022.119140
dc.identifier.issn1873-6424
dc.identifier.issn0269-7491
dc.identifier.scopus2-s2.0-85126832056
dc.identifier.urihttp://hdl.handle.net/11449/223697
dc.language.isoeng
dc.relation.ispartofEnvironmental Pollution
dc.sourceScopus
dc.subjectNeonicotinoid
dc.subjectNeotropical bee
dc.subjectOenocytes
dc.subjectStrobilurin
dc.subjectTrophocytes
dc.titleSublethal doses of imidacloprid and pyraclostrobin impair fat body of solitary bee Tetrapedia diversipes (Klug, 1810)en
dc.typeArtigo
unesp.author.orcid0000-0001-7736-4150[1]
unesp.author.orcid0000-0001-8575-2735[2]
unesp.author.orcid0000-0002-7713-6550[3]
unesp.author.orcid0000-0003-0836-8662 0000-0003-0836-8662[4]

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