Recurrent connections between Amazon and Atlantic forests shaped diversity in Caatinga four-eyed frogs

dc.contributor.authorThomé, Maria Tereza C. [UNESP]
dc.contributor.authorSequeira, Fernando
dc.contributor.authorBrusquetti, Francisco [UNESP]
dc.contributor.authorCarstens, Bryan
dc.contributor.authorHaddad, Célio F. B. [UNESP]
dc.contributor.authorRodrigues, Miguel Trefaut
dc.contributor.authorAlexandrino, João
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade do Porto
dc.contributor.institutionInstituto de Investigación Biológica del Paraguay
dc.contributor.institutionThe Ohio State University
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.abstractAim: The Caatinga is a widespread but poorly known biome in South America. Its historical stability is controversial and different types of evidence provide support to contrasting hypotheses. We investigate how past biome dynamics may have caused diversification in the endemic four-eyed frog genus Pleurodema. Location: Caatinga biome. Methods: We sampled 353 individuals of Pleurodema alium and Pleurodema diplolister from 60 localities and genotyped them at 12 (P. alium) or 20 (P. diplolister) microsatellite loci. We sequenced a mitochondrial fragment for a subset (199) of the samples. After exploratory analyses to infer genetic structure between and within species, we designed seven biogeographical scenarios based on the literature on species distributions, palaeomodels and geological palaeoindicators. We tested these hypotheses by calculating the posterior probability of models using multilocus approximate Bayesian computation (ABC). Results: Both markers recovered well-defined interspecific limits with restricted introgression, but population structure within P. diplolister is subtle and affected by isolation by distance. Model selection using ABC supported two scenarios of diversification that included recent demographic growth. Genetic breaks at intra- and interspecific levels were geographically coincident, and correlated with past forest invasions reported in the literature. Main conclusions: Our data support the idea that past expansions of the Amazon and Atlantic forests over the current Caatinga distribution shaped the genetic structure in endemic four-eyed frogs at more than one level by promoting intermittent vicariance. Additional support comes from the distribution patterns of forest and Caatinga taxa. Variation among groups suggests differential organismal responses to past habitat shifts, probably mediated by specific natural-history traits. This paper provides the first direct evidence that taxa endemic to the Caatinga were affected by past forest interactions.en
dc.description.affiliationDepartamento de Zoologia Instituto de Biociências UNESP - Universidade Estadual Paulista
dc.description.affiliationCIBIO-InBIO Centro de Investigação em Biodiversidade e Recursos Genéticos Universidade do Porto
dc.description.affiliationInstituto de Investigación Biológica del Paraguay
dc.description.affiliationDepartment of Evolution Ecology and Organismal Biology The Ohio State University
dc.description.affiliationDepartamento de Zoologia Instituto de Biociências USP - Universidade de São Paulo
dc.description.affiliationDepartamento de Ciências Biológicas UNIFESP - Universidade Federal de São Paulo
dc.description.affiliationUnespDepartamento de Zoologia Instituto de Biociências UNESP - Universidade Estadual Paulista
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFuel Cycle Technologies
dc.description.sponsorshipIdFAPESP: #2003/10335-8
dc.description.sponsorshipIdFAPESP: #2011/50146-6
dc.description.sponsorshipIdFAPESP: #2011/51392-0
dc.description.sponsorshipIdFAPESP: #2012/06611-9
dc.description.sponsorshipIdFAPESP: #2013/50741-7
dc.description.sponsorshipIdFuel Cycle Technologies: SFRH/BPD/87721/2012
dc.identifier.citationJournal of Biogeography, v. 43, n. 5, p. 1045-1056, 2016.
dc.relation.ispartofJournal of Biogeography
dc.rights.accessRightsAcesso restrito
dc.subjectChapada Diamantina
dc.subjectForest corridor
dc.subjectPast habitat shifts
dc.subjectSemi-arid biome
dc.titleRecurrent connections between Amazon and Atlantic forests shaped diversity in Caatinga four-eyed frogsen