Identification of a gene encoding adaptin-like protein in the Paracoccidioides brasiliensis genome by random amplified polymorphic DNA analysis
dc.contributor.author | Ferrari Andreotti, Patricia | |
dc.contributor.author | Monteiro da Silva, Juliana Leal | |
dc.contributor.author | Cristina Teixeira, Elaine | |
dc.contributor.author | Celia Bertolini, Maria | |
dc.contributor.author | Pienna Soares, Christiane | |
dc.contributor.author | Benard, Gil | |
dc.contributor.author | Mendes-Giannini, Maria José Soares [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2014-05-20T13:24:36Z | |
dc.date.available | 2014-05-20T13:24:36Z | |
dc.date.issued | 2007-07-01 | |
dc.description.abstract | Paracoccidioides brasiliensis isolates are not homogeneous in their patterns of pathogenicity in animals and adhesion to epithelial cells. During this investigation, genotypic differences were observed between two samples of P. brasiliensis strain 18 yeast phase (Pbl 8) previously cultured many times, one taken before (Pb18a) and the other after (Pb18b) animal inoculation. Random amplified polymorphic DNA analysis using the primer OPJ4 distinguished Pb18b from Pbl Ba by one 308 bp DNA fragment, which after cloning and sequencing was shown to encode a polypeptide sequence homologous to the protein beta-adaptin. It is suggested, by comparison to other micro-organisms, that this protein might play an important role in the virulence of P. brasiliensis. This result demonstrates the influence of in vitro subculturing on the genotype of this organism. | en |
dc.description.affiliation | UNESP, Fac Ciências Farmaceut, Dept Anal Clin, Araraquara, SP, Brazil | |
dc.description.affiliation | UNESP, Inst Quim, Dept Bioquim & Tecnol Quim, Araraquara, SP, Brazil | |
dc.description.affiliation | Univ São Paulo, Fac Med, Dept Dermatol, Lab Dermatol & Imunodeficiencias, São Paulo, Brazil | |
dc.description.affiliationUnesp | UNESP, Fac Ciências Farmaceut, Dept Anal Clin, Araraquara, SP, Brazil | |
dc.description.affiliationUnesp | UNESP, Inst Quim, Dept Bioquim & Tecnol Quim, Araraquara, SP, Brazil | |
dc.format.extent | 884-887 | |
dc.identifier | http://dx.doi.org/10.1099/jmm.0.47127-0 | |
dc.identifier.citation | Journal of Medical Microbiology. Reading: Soc General Microbiology, v. 56, n. 7, p. 884-887, 2007. | |
dc.identifier.doi | 10.1099/jmm.0.47127-0 | |
dc.identifier.issn | 0022-2615 | |
dc.identifier.lattes | 8817669953838863 | |
dc.identifier.orcid | 0000-0002-8059-0826 | |
dc.identifier.uri | http://hdl.handle.net/11449/7689 | |
dc.identifier.wos | WOS:000247759300002 | |
dc.language.iso | eng | |
dc.publisher | Soc General Microbiology | |
dc.relation.ispartof | Journal of Medical Microbiology | |
dc.relation.ispartofjcr | 2.112 | |
dc.relation.ispartofsjr | 0,914 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.title | Identification of a gene encoding adaptin-like protein in the Paracoccidioides brasiliensis genome by random amplified polymorphic DNA analysis | en |
dc.type | Artigo | |
dcterms.license | http://jmm.sgmjournals.org/site/misc/reprints.xhtml | |
dcterms.rightsHolder | Soc General Microbiology | |
unesp.author.lattes | 8817669953838863 | |
unesp.author.orcid | 0000-0002-8059-0826[7] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Química, Araraquara | pt |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquara | pt |
unesp.department | Análises Clínicas - FCF | pt |
unesp.department | Bioquímica e Tecnologia - IQ | pt |
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